皮下脂膜炎样T细胞淋巴瘤的细胞毒颗粒相关蛋白TIA—1的表达及 …

目的 认识皮下脂膜炎样Ｔ细胞淋巴瘤（ＳＰＴＣＬ）的临床和病理特点。研究细胞毒颗粒相关蛋白ＴＬＡ－１在ＳＰＴＣＬ中的表达及与Ｅｐｓｔｅｉｎ－Ｂａｒｒ（ＥＢ）病毒感染的关系。方法 和ＴＩＡ－１、ＣＤ４５ＲＯ、ＣＤ３、ＣＤ２０和ＣＤ６８等抗体作免疫组织化学ＡＢＣ法染色应用ＥＢＥＲ１／２原位杂交检测ＥＢ病的潜伏感染。结果 １７例ＳＰＴＣＬ男女之比１：１．１．中位发病年龄２４岁,主要表现为下肢、躯干无症状性

Subcutaneous panniculitis-like T-cell lymphoma: expression of cytotoxic-granule-associated protein TIA-1 and its relation with Epstein-Barr virus infection

OBJECTIVE: To study the clinicopathologic features, the expression of cytotoxic-granule-associated protein TIA-1 and Epstein-Barr virus (EBV) infection in subcutaneous panniculitis-like T-cell lymphoma (SPTCL). METHODS: Totally 17 cases of SPTCL were studied. Immunohistochemical staining was done for TIA-1, CD45RO, CD3, CD20 and CD68, and in situ hybridization for EBV encoded small nuclear RNA (EBER1/2). RESULTS: In the 17 cases of SPTCL, the ratio of male-to-female was 1:1.1 and the median age was 24 years old. The main clinical manifestations were asymptomatic plaques, nodules and masses. Histologically, the pattern of lymphoid cells infiltration was panniculitis. The infiltrated lymphoid cells were pleomorphic and their mitotic figures were few (0 - 2/hpt). In the tumor tissue, there might be epithilioid granulomas, multinuclear cells, beanbag cells, small pieces of necrosis and small vessels with lymphoid cells infiltration in the vascular wall. Immunohistochemically, all the cases showed a T-cell phenotype and the expression rate of TIA-1 was 100% (16/16). By in situ hybridization, the positive rate of EBV-EBER1/2 was 29.4% (5/17). CONCLUSIONS: SPTCL has a specific histopathologic picture. It may originate from cytotoxic T lymphocytes or NK cells. Some of them may be accompanying with EBV latent infection.