Inhibitory effects of alkyl 2-cyanoacrylate tissue adhesives on rat liver cytoplasm preparations |
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| Authors: | M B McTernan K M Hand G A Braun E A Siegmund D A Willigan |
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| Affiliation: | 1. Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210023, China;2. Affiliated Hospital of Nanjing University of TCM, Nanjing 210029, China;3. Department of Basic Medical Science, Jiangsu Vocational College of Medicine, Yanchen 224005, China;4. Nursing Department, Chemistry and Life Science College, Nanjing University Jinling College, Nanjing 210089, China;1. Department of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518060, PR China;2. Department of Chemistry, Sun Yat-sen University, Guangzhou 510275, PR China |
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| Abstract: | The in vitro effects of a homologous series of alkyl 2-cyanoacrylate monomers on the conversion of 14C-labeled acetate to respiratory 14CO2 by rat liver cytoplasm preparations were determined. The labeled preparation was added to measured amounts of monomer in vessels equipped with center wells containing p-(diisobutul cresoxyethoxyethyl) dimethylbenzylammonium hydroxide (methyl benzethonium chloride). The mixture was incubated at 37° for 45 min; metabolism was terminated with 1 n H2SO4, and the amount of 14CO2 produced was measured. The methyl homolog caused the greatest inhibition. In general, an increase of the length of the alkyl chain caused a decrease in the inhibitory effect. These results generally corroborate findings of in vivo histotoxicity studies. While this technique has been used in the past for determining pharmacologic activity, it also offers a simple and rapid means of predicting potential toxicity. |
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