Stimulation of growth of human malignant lymphoma and lymphoid leukemia cells in vitro |
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Authors: | E. -S. Strobel K. J. Bross R. Mertelsmann F. Herrmann |
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Affiliation: | (1) Department of Medicine 1 (Hematology and Oncology), University of Freiburg Medical Center, Hugstetter Strasse 55, W-7800 Freiburg, Federal Republic of Germany |
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Abstract: | Summary We have analyzed cultures of malignant lymphoma cells and cells from patients with acute lymphoid leukemia in methylcellulose for their ability to from colonies. Clonogenic growth was examined in the presence or absence of fetal calf serum (FCS), platelet-poor plasma (PPP), medium conditioned by phytohemagglutininstimulated leukocytes (PHA-LCM), or irradiated allogeneic bone marrow stroma cells. Cells from 25 lymphoma patients — 17 with non-Hodgkin's lymphoma (NHL), eight with Hodgkin's disease (HD) — and from 19 patients with acute lymphocytic leukemia (ALL) were investigated. We show that colony growth can be obtained in a minority of cases (in 3 NHL, 5 HD, and 2 ALL) and that the use of FCS and allogeneic irradiated stroma cells may be required for optimal colony formation.This work was supported by theDeutsche Forschungsgemeinschaft (Str 250/2-2). |
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Keywords: | Human lymphoma clonogenic assay Human bone marrow stroma |
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