Mechanisms underlying diabetes enhancement of endothelin-1-induced contraction in rabbit basilar artery |
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Authors: | Alabadí José A Miranda Francisco J Lloréns Silvia Centeno José M Marrachelli Vannina G Alborch Enrique |
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Affiliation: | Departamento de Fisiología, Facultad de Farmacia, Universitat de València, Avda Vicent Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain. Jose.A.Alabadi@uv.es |
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Abstract: | The influence of alloxan-induced diabetes on the reactivity of rabbit basilar artery to endothelin-1 was examined. Endothelin-1 induced concentration-dependent contraction of basilar arteries that was higher in diabetic than in control rabbits. Endothelium removal produced a higher enhancement of the endothelin-1-induced contraction in control than in diabetic rabbits. N(G)-nitro-L-arginine (L-NOArg) enhanced the maximal contraction induced by endothelin-1 in control rabbits and potentiated this response in diabetic rabbits. Endothelin ETA receptor antagonist, cyclo(D-Asp-Pro-D-Val-Leu-D-Trp) (BQ-123), inhibited endothelin-1-induced contraction in both rabbit groups. Endothelin ETB receptor antagonist, 2,6-Dimethylpiperidinecarbonyl-gamma-Methyl-Leu-Nin-(Methoxycarbonyl)-D-Trp-D-Nle (BQ-788), enhanced endothelin-1-induced contraction in control rabbits and decreased the potency of endothelin-1 in diabetic rabbits. Sodium nitroprusside-induced relaxation of basilar arteries was lower in diabetic than in control rabbits. These results suggest that mechanisms underlying rabbit basilar artery hyperreactivity to endothelin-1 include decreased endothelial modulation of endothelin-1-induced contraction, with impaired endothelial endothelin ETB receptor activity; decreased sensitivity to nitric oxide (NO) in vascular smooth muscle; and enhanced participation of muscular endothelin ETA and ETB receptors. |
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Keywords: | Diabetes Endothelin-1 Endothelium NO (Nitric oxide) Basilar artery (Rabbit) |
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