Diphenylene iodonium, an inhibitor of free radical formation, inhibits platelet aggregation. |
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Authors: | D Salvemini W Radziszewski V Mollace A Moore D Willoughby J Vane |
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Institution: | William Harvey Research Institute, St. Bartholomew's Medical College, London, U.K. |
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Abstract: | Diphenylene iodonium is an inhibitor of the enzyme NADPH-oxidase and prevents the generation of oxygen-derived free radicals in neutrophils (Cross and Jones, 1986). Here we show that diphenylene iodonium (0.25-2 microM) inhibited, according to the dose, thrombin-induced platelet-aggregation in human washed platelets and ADP-induced platelet aggregation in platelet-rich plasma. At the concentrations which inhibited platelet aggregation diphenylene iodonium did not alter platelet concentrations of cAMP or cGMP but enhanced the anti-platelet activity of iloprost, sodium nitroprusside or cultured endothelial cells. These findings highlight the importance of free radicals as platelet pro-aggregatory agents. |
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