MMP-2, MMP-9 and their inhibitors TIMP-2 and TIMP-1 production by human monocytes in vitro in the presence of different forms of hydroxyapatite particles |
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Authors: | Laquerriere Patrice Grandjean-Laquerriere Alexia Addadi-Rebbah Salima Jallot Edouard Laurent-Maquin Dominique Frayssinet Patrick Guenounou Moncef |
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Institution: | Laboratoire de Microscopie Electronique, INSERM ERM 0203, IFR 53, UFR Sciences, 21, Rue Clément Ader, BP 138, Cedex 2, 51685 Reims, France. patrice.laquerriere@univ-reims.fr |
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Abstract: | After calcium-phosphates biomaterials based implantation like hydroxyapatite (HA) coating, particles are released in the periprosthetic tissues. Wear-debris induced fibrous membranes contain macrophage subsets that can produce metalloproteinases (MMPs), which are considered to be key enzymes in extra-cellular matrix turnover. Tissue inhibitors of metalloproteinases (TIMPs) are important regulator of MMPs activity. Interleukin-1 mainly produced by monocytes can also regulate MMPs production. In the present work, we have evaluated the effect of HA particles characteristics (size, shape and sintering temperature) on the MMP-2, -9 and their respective inhibitors TIMP-2, -1 production. Our results demonstrate that sintering temperature (that modify crystal size and surface area) have little effect on MMPs and TIMPs production. Non-phagocytable particles induced more MMP-9, although phagocytable particles induced more IL-1beta release. The shape of the particles was the most important factor since needle-shaped particles induced the most significant up-regulated expression of MMPs and IL-1beta. |
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