母乳巨细胞病毒感染状况研究

目的；探讨母乳巨细胞病毒感染状况及意义。方法；采用酶联免疫吸附试验（ELISA）测定乳清HCMV-IgM特异性抗体；多聚酶链反应（PCR）技术检测乳清HCMV-DNA。结果：农村哺乳期妇女HCMV-IgM和HCMV-DNA阳性率（18．1％和21．0％）皆高于城区（8．3％和11．2％）；成熟乳HCMV-DNA阳性率（20．4％）高于初乳（11．5％）。结论：母乳巨细胞病毒感染是婴幼儿获得性巨细胞病毒感染的重要途径。对早产儿和免疫缺陷儿，不易采用感染的母乳喂养，对感染的母亲及其哺乳的正常婴儿，应定期进行随访观察。

A STUDY ON HCMV INFECTION STATUS OF BREAST MILK.

Objective:To assess the status and colinical significance lof human cytomegalovirus (HCMV) infetion of breast milk.Methods;Specific HCMV-IgM in human whey was detected by enzyme linked immunosorbent assay(ELISA);HCMV-DNA in human whey was determined by polymerase chain reation (PCR).Results:The positive rates of HCMV-IgM and HCMV-DNA(18.1% and 21.0%) of breast-feeding women in rural areas were significantly higher than that(8.3%)and 11.2%)in ruban areas.?The polsitive rate of HCMV-DAN(20.4%) in late milk was significantly higher than that(11.5%)in colostrum.Conclusion:Breast milk with HCMV infection may be a important channel of acquird HCMV infection in infants In-fected breast-milk is not fit to feed premature and immunodeficiency infants.Infected mother and infant fed with her breast-milk should regolarly follow up.