大孔吸附树脂纯化葛根总皂苷的研究

目的筛选大孔吸附树脂纯化葛根总皂苷的最佳工艺,为葛根工业化生产提供参考。方法以葛根总皂苷质量分数为指标,从树脂类型、上样吸附速度、洗脱剂用量、乙醇体积分数等参数优选葛根总皂苷的最佳纯化工艺条件。结果 D101型大孔吸附树脂纯化效果较好,其最佳工艺条件为:取适当质量浓度的供试品溶液上样,吸附速度为2 BV.h-1,吸附饱和后,先以4 BV水洗脱,再分别以体积分数30%、50%及70%乙醇各4 BV洗脱。纯化后产物中总皂苷质量分数为26.32%。结论本方法简便可行、纯化效果好,可为葛根复方新药研发及工业化生产提供参考。

Study on purification of total saponins from Pueraria lobata with macroporons resin

Objective To investigate the purification of total saponins from Pueraria lobata by macroporous resin to provide evidence for the industrial production.Methods A reasonable purifying process was established,and the resin’s specifications,the flow rate of the sample’s adsorption,the amount of the eluant and,the concentrations of ethanol,and so on,were investigated with the yields of total saponins as index.Results D101 resin showed a good purifying profile in the conditions as follows: adsorbing to saturation at 2 BV·h-1,then eluting successively with distilled water,30%,50% and 70% ethanol,for 4 BV respectively.The yielding of total saponins was 26.32%.Conclusion It’s a reliable and accurate method,which may be suitable for the development and industrial production of saponins from Pueraria lobata.