救必应皂苷类成分的组织化学研究

目的应用植物解剖学和组织化学技术,研究救必应皂苷成分的累积分布状态。方法将救必应不同部位的显微切片用5%(质量浓度)香草醛-冰醋酸和高氯酸混合试剂进行染色后,放置于显微镜下观察并拍摄显微图片,阴性对照为用FAA固定液浸泡1个月后的组织切片。结果救必应皂苷成分在根中主要分布于次生韧皮部和皮层薄壁细胞中;在茎中主要分布于次生韧皮部细胞中,少量分布在韧皮部;在叶中则主要分布在栅栏组织中,海绵组织有少量分布。结论救必应中的皂苷成分主要分布在营养器官的薄壁组织细胞中。

Histochemiscal study on the saponins from Ilex rotunda Thunb.

Objective To investigate the localization of saponins with anatomical and histochemical methods in Ilex rotunda Thunb..Methods Different cross-sections of I.rotunda were dyed with mixed 5% vanillin-glacial acetic acid and perchloric acid,then observed in a microscope and photographed.The negative control product was the biopsy tissue socked with FAA for 1 month.Results Histochemistry results showed that saponins distributed in secondary phloem and parenchyma cells of root cortex,in secondary phloem and a little in phloem of stem,mainly in palisade tissue and a little in spongy tissue of leaf.Conclusion Saponins accumulated mainly in parenchyma cells of vegetative organs of I.rotunda.