动脉粥样硬化患者外周血单个核细胞CD54表达的初步研究

目的探讨动脉粥样硬化和细胞间粘附分子－１（ＩＣＡＭ－１，即ＣＤ５４）间的关系。方法 用ＡＰＡＡＰ桥联酶免疫细胞化学染色法检测冠心病病人ＣＤ５４阳性单个核细胞百分率。结果 冠心病病人ＣＤ５４阳性率显著增高，正常人单个核细胞经内毒素，人高水平ＬＤＬ血清处理后增高的ＣＤ５４阳性率可达ＣＨＤ病人水平，经凝血酶处理后ＣＤ５４性率无显著变化，而内毒素还可使ＣＨＤ病人单个核细胞表达ＣＤ５４再度增多。结论 提示单

Study of CD54 expression on mononuclear cells in peripheral blood of patients with atherosclerosis

Objective To explore the relationship between intercellular adhesion molecule - 1 (ICAM - 1, CD54) on mononuclear cells (MCs) and atherosclerosis (As). Methods CD54 on MCs were detected by APAAP immunocytochemical staining in patients with coronary heart disease (CHD). The percentage of positive cell were counted. Results The percentage of CD54 on MCs in patients was significantly higher than that in normal persons. MCs from normal persons treated with endotoxin or serum with high level of LDL, CD54 positive MCs were signifi- cantly increased, but treated with thrombin, CD54 positive MCs were not increased, MCs from patients treated with endotoxin, the percentage of CD54 was also increased. Conclusion It is probable that increased expression of CD54 on membrane of MCs is associated with occurrence and progression of As.