| 慢病毒介导YCD/5-FC自杀基因系统对白血病Jurkat细胞杀伤和旁观者效应 |
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| 引用本文： | 侯军,陈洁,李萍萍,陶怡,周虹,章卫平,王健民. 慢病毒介导YCD/5-FC自杀基因系统对白血病Jurkat细胞杀伤和旁观者效应[J]. 中国肿瘤生物治疗杂志, 2011, 18(1): 17-22. DOI: 10.3872/J.issn.1007-385X.2011.01.004 |
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| 作者姓名： | 侯军 陈洁 李萍萍 陶怡 周虹 章卫平 王健民 |
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| 作者单位： | 第二军医大学,长海医院,血液科,上海,200433 |
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| 基金项目： | 国家自然科学基金资助项目（No. 30871100，No.30172347）；上海市科委重点项目基金资助（No.8JC1406500） |
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| 摘 要： | 目的:探讨慢病毒介导的酵母菌胞嘧啶脱氨酶(yeast cytosine deaminase,YCD)自杀基因对人白血病Jurkat细胞的杀伤作用和旁观者效应。方法:制备YCD-GFP慢病毒上清,转染Jurkat细胞(即Jurkat/YCD-GFP细胞),流式细胞术检测YCD-GFP基因的表达。体外观察前体药物5-氟胞嘧啶(5-fluorocytosine,5-FC)对Jurkat/YCD-GFP细胞的杀伤情况和旁观者效应;建立Jurkat/YCD-GFP荷瘤小鼠模型,体内观察5-FC对移植瘤细胞的杀伤作用和旁观者效应。以上实验均以Jukut/GFP细胞为对照。结果:成功制备YCD-GFP慢病毒和感染YCD-GFP慢病毒的Jurkat/YCD-GFP细胞,YCD-GFP慢病毒对Jurkat细胞的感染率达96.93%。232μmol/L的5-FC作用72h可使(95.61±2.07)%的Jurkat/YCD-GFP细胞死亡,且上清中5-FC含量下降80%左右。体外实验显示,Jurkat/YCD-GFP和Jurkat细胞效靶比1∶10混合培养[(68.69±4.97)%vs(97.87±1.11)%,P<0.05)]和Transwell1∶25分层培养[(1.46±0.27)×105vs(3.00±0.16)×105,P<0.01]均存在旁观者效应。体内实验显示,YCD/5-FC自杀基因系统对Jurkat/YCD-GFP荷瘤小鼠亦具有较强的杀伤作用和旁观者效应(P<0.05)。结论:慢病毒介导的YCD/5-FC自杀基因系统对人Jurkat白血病细胞具有显著的杀伤作用和旁观者效应。
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| 关 键 词： | YCD/5-FC 自杀基因 慢病毒载体 白血病 Jurkat细胞 旁观者效应 |
| 收稿时间： | 2010-11-08 |
| 修稿时间： | 2010-12-18 |
Cytotoxicity and bystander effect of lentiviral vector-mediated YCD/5-FC suicide gene system against leukemia Jurkat cells |
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| HOU Jun,CHEN Jie,LI Ping-ping,TAO Yi,ZHOU Hong,ZHANG Wei-ping and WANG Jian-min. Cytotoxicity and bystander effect of lentiviral vector-mediated YCD/5-FC suicide gene system against leukemia Jurkat cells[J]. Chinses Journal of Cancer Biotherapy, 2011, 18(1): 17-22. DOI: 10.3872/J.issn.1007-385X.2011.01.004 |
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| Authors: | HOU Jun CHEN Jie LI Ping-ping TAO Yi ZHOU Hong ZHANG Wei-ping WANG Jian-min |
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| Affiliation: | Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China |
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| Abstract: | |
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| Keywords: | YCD/5-FC suicide gene lentiviral vector leukemia Jurkat cell bystander effect |
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