| 流式细胞术检测药物抗菌效应方法学的建立 |
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| 引用本文： | 李庆,马筱玲,翟志敏,周珞平. 流式细胞术检测药物抗菌效应方法学的建立[J]. 安徽医学, 2003, 24(3): 8-10 |
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| 作者姓名： | 李庆 马筱玲 翟志敏 周珞平 |
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| 作者单位： | 230001,合肥,安徽省立医院检验科;230001,合肥,安徽省立医院中心实验室 |
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| 基金项目： | 安徽省科技攻关项目;01013039; |
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| 摘 要： | 目的 尝试建立用流式细胞术检测药物抗菌效应的新方法。方法 以碘化丙啶为荧光染料 ,用流式细胞术检测大肠埃希菌标准菌株ATCC 2 5 92 2在不同浓度头孢噻肟作用下各管平均荧光强度 ,并就最佳实验条件进行探讨。结果 细菌平均荧光强度值随着抗生素浓度的增加而增强 ,其变化具有浓度依赖性 ,较好地反映了药物的抗菌效应。结论 成功建立用流式细胞术检测药物抗菌效应的新方法 ,流式细胞术在该领域的应用 ,值得我们做进一步研究
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| 关 键 词： | 流式细胞术 大肠埃希菌 头孢噻肟 抗菌效应 |
The development of methodology for drug anti-bacterial effect by flow cytometry |
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| Li Qing,Ma Xiaoling,Zhai Zhimin,et al.. The development of methodology for drug anti-bacterial effect by flow cytometry[J]. Anhui Medical Journal, 2003, 24(3): 8-10 |
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| Authors: | Li Qing Ma Xiaoling Zhai Zhimin et al. |
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| Affiliation: | Li Qing,Ma Xiaoling,Zhai Zhimin,et al.Department of Clinical Laboratory,Central Laboratory,Anhui Provincial Hospital,Hefei 230001 |
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| Abstract: | Objective To develop a new method for druganti?bacterial effect by flow cytometry(FCM).Methods ATCC 25922 of E.coli were fluorescence labeled by propidium iodide(PI),mean fluorescence intensity (MFI)under different durg concentrations were detected by flowcytometry,and explore the best experiment condition.Results With drug concentrains rising,the MFI of bacteria were increasing,the changetrends is concentration?dependent, which reflect the drug antibacterial effectexactly.Conclusion It was successful to develop a new method for drug anti?bacterial effect by flow cytometry,the application of FCM inthis field is worth studying it deeply. |
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| Keywords: | Flow cytometry Escherchia coli Cetotaxime Anti?bacterial effect |
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