| PEG10 Activation by Co-Stimulation of CXCR5 and CCR7 Essentially Contributes to Resistance to Apoptosis in CD19^＋CD34^＋ B Cells from Patients with B Cell Lineage Acute and Chronic Lymphocytic Leukemia |
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| 引用本文： | Hu C,Xiong J,Zhang L,Huang B,Zhang Q,Li Q,Yang M,Wu Y,Wu Q,Shen Q,Gao Q,Zhang K,Sun Z,Liu J,Jin Y,Tan J. PEG10 Activation by Co-Stimulation of CXCR5 and CCR7 Essentially Contributes to Resistance to Apoptosis in CD19^＋CD34^＋ B Cells from Patients with B Cell Lineage Acute and Chronic Lymphocytic Leukemia[J]. Cellular & molecular immunology, 2004, 1(4): 280-294 |
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| 作者姓名： | Hu C Xiong J Zhang L Huang B Zhang Q Li Q Yang M Wu Y Wu Q Shen Q Gao Q Zhang K Sun Z Liu J Jin Y Tan J |
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| 作者单位： | Chunsong Hu~1 Jei Xiong~(2,3) Linjei Zhang~1 Baojun Huang~1 Qiuping Zhang~3 Qun Li~1 Mingzhen Yang~4 Yaou Wu~1 Qun Wu~3 Qian Shen~1 Qingping Gao~5 Kejian Zhang~5 Zhimin Sun~6 Junyan Liu~3 Youxin Jin~2 Jinquan Tan~(1,3,7) ~1Department of Immunology,College of Basic Medical Sciences.Anhui Medical University,Hefei 230032,China.~2The Stat Key Laboratory of Molecular Biology.Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences.Chinese Academy of Science,Shanghai,China.~3Department of Immunology,and Sino-Danish Joint Laboratory of Immunology.Wuhan University School of Medicine.Wuhan 430071.China.~4Department of Hematology,The Affiliated University Hospital.Anhui Medical University,Hefei 230031.China.~5Department of Hematology,The Renmin and Zongnan University Hospital,Wuhan University,Wuhan 43007 1,China.~6Department of Hematology.The Provincial Hospital of Anhui.Hcfei 230020.China. |
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| 摘 要： | We investigated CD19~+CD34~+ and CD19~+CD34 B cells from cord blood (CB) and typical patients with B celllineage acute and chronic lymphocytic leukemia (B-ALL and B-CLL) in terms of expression and functions ofCXCR5/CXCL13 and CCR7/CCL19.CXCR5 and CCR7 were selectively frequent expressed on B-ALL,B-CLLand CB CD19~+CD34~+ B cells,but not on CD19~+CD34 B cells.Instead of induction of impressive chemotacticresponsiveness,CXCL13 and CCL19 together induced significant resistance to TNF-α-mediated apoptosis inB-ALL and B-CLL but not CB CD19~+CD34~+ B cells.B-ALL and B-CLL CD19~+CD34~+ B cells expressed elevatedlevel of Paternally Expressed Gene 10 (PEG10),and CXCL13 and CCL19 together significantly up-regulatedPEG10 expression in the cells.We found that CXCL13 and CCL19 together by means of activation of CXCR5and CCR7 up-regulated PEG10 expression and function,subsequent stabilized caspase-3 and caspase-8 inB-ALL and B-CLL CD19~+CD34~+ B cells,and rescued the cells from TNF-α-mediated apoptosis.We suggestedthat normal lymphocytes,especially naive B and T cells,utilized CXCR5/CXCL13 and CCR7/CCL19 formigration,homing,maturation,and cell homeostasis as well as secondary lymphoid tissues organogenesis.Meanwhile certain malignant cells took advantages of CXCR5/CXCL13 and CCR7/CCL19 for infiltration,resistance to apoptosis,and inappropriate proliferation.Cellular & Molecular Immunology.2004;1(4):280-294.
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| 关 键 词： | PEG10 活化作用 Co-刺激 CXCR5 CCR7 抵抗力 细胞凋亡 CD19^＋CD34^＋B细胞 B细胞血统 急性期 慢性淋巴系白血病 趋药性 |
PEG10 activation by co-stimulation of CXCR5 and CCR7 essentially contributes to resistance to apoptosis in CD19+CD34+ B cells from patients with B cell lineage acute and chronic lymphocytic leukemia |
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| Hu Chunsong,Xiong Jei,Zhang Linjei,Huang Baojun,Zhang Qiuping,Li Qun,Yang Mingzhen,Wu Yaou,Wu Qun,Shen Qian,Gao Qingping,Zhang Kejian,Sun Zhimin,Liu Junyan,Jin Youxin,Tan Jinquan. PEG10 activation by co-stimulation of CXCR5 and CCR7 essentially contributes to resistance to apoptosis in CD19+CD34+ B cells from patients with B cell lineage acute and chronic lymphocytic leukemia[J]. Cellular & molecular immunology, 2004, 1(4): 280-294 |
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| Authors: | Hu Chunsong Xiong Jei Zhang Linjei Huang Baojun Zhang Qiuping Li Qun Yang Mingzhen Wu Yaou Wu Qun Shen Qian Gao Qingping Zhang Kejian Sun Zhimin Liu Junyan Jin Youxin Tan Jinquan |
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| Affiliation: | Department of Immunology, College of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China. |
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| Abstract: | We investigated CD19+CD34+ and CD19+CD34- B cells from cord blood (CB) and typical patients with B cell lineage acute and chronic lymphocytic leukemia (B-ALL and B-CLL) in terms of expression and functions of CXCR5/CXCL13 and CCR7/CCL19. CXCR5 and CCR7 were selectively frequent expressed on B-ALL, B-CLL and CB CD19+CD34+ B cells, but not on CD19+CD34- B cells. Instead of induction of impressive chemotactic responsiveness, CXCL13 and CCL19 together induced significant resistance to TNF-alpha-mediated apoptosis in B-ALL and B-CLL but not CB CD19+CD34+ B cells. B-ALL and B-CLL CD19+CD34+ B cells expressed elevated level of Paternally Expressed Gene 10 (PEG10), and CXCL13 and CCL19 together significantly up-regulated PEG10 expression in the cells. We found that CXCL13 and CCL19 together by means of activation of CXCR5 and CCR7 up-regulated PEG10 expression and function, subsequent stabilized caspase-3 and caspase-8 in B-ALL and B-CLL CD19+CD34+ B cells, and rescued the cells from TNF-alpha-mediated apoptosis. We suggested that normal lymphocytes, especially naive B and T cells, utilized CXCR5/CXCL13 and CCR7/CCL19 for migration, homing, maturation, and cell homeostasis as well as secondary lymphoid tissues organogenesis. Meanwhile certain malignant cells took advantages of CXCR5/CXCL13 and CCR7/CCL19 for infiltration, resistance to apoptosis, and inappropriate proliferation. |
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| Keywords: | leukemia B cells chemokine receptor apoptosis chemotaxis |
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