| RASSF1B基因的克隆及序列分析 |
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| 引用本文： | 陈吉祥,陈明军,张尤历. RASSF1B基因的克隆及序列分析[J]. 山东医药, 2010, 50(10): 8-9 |
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| 作者姓名： | 陈吉祥 陈明军 张尤历 |
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| 作者单位： | 江苏大学附属医院,江苏镇江,212001 |
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| 基金项目： | 江苏省镇江市科技发展基金资助项目 |
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| 摘 要： | 目的克隆RASSF1B基因并观察其序列。方法采用RT-PCR从胃腺癌细胞AGS、肝癌细胞HEPG2、高转移肺癌细胞95D、肺腺癌细胞LTEP—a-2、白血病细胞U937中克隆RASSF1B基因，用生物信息学方法对克隆基因的序列进行分析。结果RASSF1B在5种肿瘤细胞中均有表达。序列分析显示，该基因中有一个Ras结合区域和一个SARAH区域。结论在胃腺癌、肝癌、高转移肺癌、肺腺癌、白血病细胞中成功克隆出RASSF1B基因，该基因序列中含有一个Ras结合区域和一个SARAH区域。
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| 关 键 词： | RASSF1B基因 基因克隆 寡核苷酸序列分析 |
Clone and sequence analysis of RASSF1B gene |
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| CHEN Ji-xiang,CHEN Ming-jun,ZHANG You-li. Clone and sequence analysis of RASSF1B gene[J]. Shandong Medical Journal, 2010, 50(10): 8-9 |
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| Authors: | CHEN Ji-xiang CHEN Ming-jun ZHANG You-li |
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| Affiliation: | CHEN Ji-xiang,CHEN Ming-jun,ZHANG You-li(Affiliated Hospital,Jiangsu University,Zhenjiang212001,P.R.China) |
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| Abstract: | Objective To clone the RASSF1 gene and observe its sequence.Methods The RASSF1 gene was cloned in gastric cancer cell AGS,liver cancer cell HEPG2,highly metastatic lung cancer cell 95D,lung adenocarcinoma cell LTEP-a-2,leukemia cell U937 by RT-PCR,its sequence was analized by biological information method.Results The RASSF1B gene expressed in all tested tumor cells.A Ras protein binding domain and an SARAH region were observed in the RASSF1B gene.Conclusions The RASSF1B gene is cloned successfully in gastri... |
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| Keywords: | RASSF1 gene gene clone oligonucleotide array sequence analysis |
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