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| 罗格列酮对D-氨基半乳糖联合脂多糖诱导的小鼠急性肝衰竭的保护作用 | |
| 引用本文： | 陈海鸥,谢玉桃,傅爱.罗格列酮对D-氨基半乳糖联合脂多糖诱导的小鼠急性肝衰竭的保护作用[J].胃肠病学和肝病学杂志,2010,19(3). |
| 作者姓名： | 陈海鸥 谢玉桃 傅爱 |
| 作者单位： | 1. 湖南省人民医院肝病内科 2. 中南大学湘雅医院感染病科,湖南,长沙,410008 3. 长沙市中心医院胸科中心 |
| 摘 要： | 目的观察罗格列酮对D-氨基半乳糖(D-GalN)联合脂多糖(LPS)诱导的小鼠急性肝衰竭的保护作用,并研究其可能的作用机制。方法雄性昆明小鼠随机分为三组:正常组、对照组和治疗组。对照组和治疗组以D-GalN/LPS腹腔注射构建小鼠急性肝衰竭模型,正常组则相应予以生理盐水腹腔注射;治疗组于造模前2 h予以罗格列酮灌胃,正常组和对照组则相应予以生理盐水灌胃。比较各组小鼠24 h存活率,检测血清丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)水平,肝组织病变程度及肝组织中肿瘤坏死因子-α(TNF-α)和天冬氨酸特异半胱氨酸蛋白酶-3(Caspase-3)mRNA的表达水平。结果治疗组小鼠24 h存活率明显高于对照组(P0.05),血清ALT、AST水平明显低于对照组(P0.05);治疗组与对照组比较,肝组织炎性细胞浸润明显减少,肝细胞以变性为主,未见明显坏死;治疗组小鼠肝组织中TNF-α、Caspase-3 mRNA表达水平明显低于对照组(P0.05)。结论罗格列酮对D-GalN/LPS小鼠急性肝衰竭有保护作用,可改善肝细胞炎症和坏死,降低急性肝衰竭的死亡率,其机制可能与罗格列酮下调TNF-α、Caspase-3的表达有关。 |
| 关 键 词： | 急性肝衰竭 罗格列酮 天冬氨酸特异半胱氨酸蛋白酶-3 肿瘤坏死因子-α |
The protective effect of Rosiglitazone on mice acute hepatic failure induced by D-GalN and LPS |
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| CHEN Haiou,XIE Yutao,FU Ai.The protective effect of Rosiglitazone on mice acute hepatic failure induced by D-GalN and LPS[J].Chinese Journal of Gastroenterology and Hepatology,2010,19(3). | |
| Authors: | CHEN Haiou XIE Yutao FU Ai |
| Institution: | CHEN Hai\'ou1,XIE Yutao1,FU Ai21.Department of Infectious Disease,Xiangya Hospital,Central South University,Changsha 410008,2.Department of Tuberculosis,Central Hospital of Changsha,China |
| Abstract: | Objective To observe the protective effect of Rosiglitazone on the mice acute hepatic failure induced by D-GalN and LPS,and to explore the possible mechanism.Methods Male mice were randomly divided into three groups: normal group,control group and therapy group.The control group and the therapy group were intraperitoneally injected by D-GalN/LPS,while the normal group was injected by saline;the therapy group was intragastircally lavaged by Rosiglitazone at 2 hours before the D-GalN/LPS injection,while the c... |
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