Phoenix-100全自动微生物鉴定/药敏系统检测肠球菌方法评估

目的用Phoen ix-100全自动微生物鉴定/药敏系统(简称Phoen ix-100系统)检测肠球菌并评估此检测系统。方法收集50株临床分离肠球菌,用Phoen ix-100系统进行鉴定及药敏试验,通过与API鉴定系统、药敏纸片法和E-test法[筛选耐万古霉素肠球菌(VRE)]比较,对Phoen ix-100系统进行评估。结果与API鉴定系统相比,Phoen ix-100系统鉴定肠球菌的一致率为90.0%。与药敏纸片法相比,Phoen ix-100系统检测肠球菌对庆大霉素(筛选耐高浓度氨基糖苷类抗生素肠球菌)、氨苄西林、环丙沙星、红霉素、呋喃妥因、万古霉素和肽可霉素药敏结果的完全符合率分别为94.0%、94.0%、88.0%、94.0%、70.8%、64.0%和92.0%。与E-test法相比,Phoen ix-100系统筛选VRE的完全符合率为84.0%;未出现极大错误,大错误率为12.0%,小错误率为4.0%。结论用Phoen ix-100系统检测肠球菌是一种简便、快速和比较准确可靠的方法。

Evaluation of the Phoenix-100 automated microbiology system for identification and antimicrobial susceptibility testing of Enterococci

Objective To evaluate the performance of the Phoenix-100 automated microbiology system for identification(ID) and antimicrobial susceptibility testing(AST) of Enterococci. Methods 50 clinical isolates of Enterococci were collected.All isolates were identified and detected AST by the Phoenix-100 system.ID results from Phoenix-100 were compared with those of API 20 STREP,the disk diffusion method(K-B) and the E-test method [for screening vancomycin resistant Enterococci(VRE) only].Results Concordant ID of 90.0% was observed for Enterococci between Phoenix-100 and API.Compared with K-B,the category agreements of Phoenix-100 for gentamicin for screening high level aminoglycoside resistant(HLAR),ampicillin,ciprofloxacin,erythromycin,nitrofurantoin,vancomycin,and teicoplanin were 94.0%,94.0%,88.0%,94.0%,70.8%,64.0% and 92.0% respectively;Compared with E-test,the category agreement,very major error rate,major error rate,and minor error rate of screening VRE for Phoenix-100 were 84.0%,0,12.0% and 4.0% respectively. Conclusions The Phoenix-100 ID results show high agreement with the API results;The AST performance is equivalent to the methods K-B and E-test.It is an easy,rapid,essentially accurate and reliable method to detect Enterococci by the Phoenix-100 system.