| 反义p53寡核苷酸对阿霉素诱导的培养人皮肤角质形成细胞凋亡的影响 |
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| 引用本文： | 张兴洪,刘彦群,田美华.反义p53寡核苷酸对阿霉素诱导的培养人皮肤角质形成细胞凋亡的影响[J].中国中西医结合皮肤性病学杂志,2011,10(3):141-144. |
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| 作者姓名： | 张兴洪 刘彦群 田美华 |
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| 作者单位： | 徐州医学院附属医院,徐州,221002 |
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| 基金项目： | 江苏省中医药局中医药科研项目 |
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| 摘 要： | 目的 探讨反义p53寡核苷酸(ODN)对阿霉素诱导的培养人皮肤角质形成细胞凋亡的影响.方法 用MTT法检测不同浓度脂质体和0.5mg/L反义p53 ODN转染角质形成细胞后对2mg/L阿霉素抑制细胞增殖的影响;RT-PCR方法 测定p53 mRNA水平的变化;用流式细胞仪检测细胞凋亡.结果 阿霉素抑制体外培养的人皮肤角...
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| 关 键 词： | p53 反义寡核苷酸 阿霉素 角质形成细胞 细胞凋亡 |
Effect of Antisense p53 Oligodeoxynucleotides on Adriamycin Inducing Apoptosis of Cultured Human Skin Keratinocytes in Vitro |
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| ZHANG Xing-hong,LIU Yan-qun,Tian Mei-hua.Effect of Antisense p53 Oligodeoxynucleotides on Adriamycin Inducing Apoptosis of Cultured Human Skin Keratinocytes in Vitro[J].Chinese J of Dermatovenerology Integr Tradit and West Med,2011,10(3):141-144. |
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| Authors: | ZHANG Xing-hong LIU Yan-qun Tian Mei-hua |
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| Institution: | ZHANG Xing-hong,LIU Yan-qun,Tian Mei-hua Affiliated Hospital of Xuzhou Medical College,Xuzhou 221002,China |
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| Abstract: | Objective To investigate the effect of antisense p53 oligodeoxynucleotides(ODN) on adriamycin inducing apoptosis of cultured human skin keratinocytes in vitro.Methods Human skin keratinocytes were cultured with 2mg/L adriamycin and transfected with 0.5mg/L antisense p53 ODN and different concentrations of liposome.Cell proliferation was detected by colorimetric assay of MTT.Then the optical density values were measured on a scanning multiwell spectrophotometer(ELISA reader).The mRNA of p53 was also observed... |
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| Keywords: | p53 |
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