电针天枢穴对重症急性胰腺炎大鼠血清促炎和抗炎因子的影响

目的：探讨电针天枢穴对牛黄胆酸钠诱导的重症急性胰腺炎（severe acute pancreatitis,SAP）大鼠血清促炎和抗炎因子的影响。方法：将66只SD雄性大鼠随机分为假手术组、模型组、电针组,每组22只。通过胆胰管注射3.5%牛黄胆酸钠制作SAP模型。电针组分别在模型制作成功后和处死前给予电针天枢穴治疗各30min（2/100Hz,2mA）。各组大鼠分别于术后3、6和12h分批处死,留取血液及胰腺组织标本,酶联免疫吸附法检测各组血清中肿瘤坏死因子α（tumor necrosis factor-α,TNF-α）、白细胞介素6（interleukin-6,IL-6）和白细胞介素10（interleukin-10,IL-10）的浓度,苏木精和伊红染色观察各组胰腺组织病理变化并进行评分。结果：在术后3、6和12h时模型组血清TNF-α、IL-6浓度和3、6h时的IL-10浓度均高于假手术组（P〈0.05）。术后6和12h时,电针组血清TNF-α、IL-6浓度较模型组明显降低,IL-10浓度、IL-10/TNF-α浓度比值较模型组升高（P〈0.05）,胰腺组织病理学评分较模型组明显降低（P〈0.05）。结论：电针天枢穴可以减轻SAP大鼠胰腺病理损伤,其作用机制可能是通过下调TNF-α、IL-6等促炎细胞因子的释放,并一定程度提高抗炎细胞因子IL-10水平,重建促炎和抗炎细胞因子的平衡,减轻SAP炎症的进一步发展。

Effects of electroacupuncture at Tianshu (ST25) on pro- and anti-inflammatory cytokines in rats with severe acute pancreatitis

Objective:To observe the effects of electroacupuncture(EA)at Tianshu(ST25)on pro-and anti-inflammatory cytokines in sodium taurocholate-induced severe acute pancreatitis(SAP)in rats.Methods:Sixty-six male Sprague-Dawley rats were randomly divided into three groups:sham-operation group,SAP group and EA group.An SAP model was established by injecting 3.5% sodium taurocholate at a dose of 1 mL/kg through puncturing the pancreatic duct.In the EA group,EA at ST25 was performed with sparse-dense wave(2/100 Hz,2 m...