不同处理的人血清白蛋白对人牙龈上皮细胞粘附作用的影响

目的：探讨人血清白蛋白（human serum albumin,HSA)对人牙龈上皮细胞（human gingival epithelial cells,HGE)粘附作用的影响。方法：使用角化细胞无血清培养基、分离酶原代培养HGE并用广谱细胞角蛋白单抗作细胞鉴定，细胞接种24h内MTT法连续观测HSA聚苯乙烯表面预孵育组、培养基含HSA组HGE的粘附，在含HSA培养基中观察HGE的生长曲线。结果：HGE免疫组化染色阳性。HSA预孵育组，8h以内粘附的细胞数量显著少于培养基含HSA组及对照组，10h至24h两实验组与对照组无显著性差异；生长曲线中各时间点细胞的数量，实验组与对照组相比无显著性差异。结论：HSA预孵育于聚苯乙烯表面，对HGE的粘附在早期产生抑作用。

Effect of Human Serum Albumin of Different Employment on Cell Attachment of Human Gingival Epithelial Cells

Objective: To investigate the effect of human serum albumin (HSA) of different employment on cell attachment of human gingival epithelial cells (HGE). Methods: HGE were primarily cultured with keratinocyte serum free medium (KSFM) and dispase. The cultured cells were immunohistochemical stained by monoclonal anti pan cytokeratin. MTT test was employed to investigate the influence of HSA on the cell attachment on polystyrene surface. The cell growth curve of HGE which were cultured in KSFM with 50 mg/ml HSA was observed. Results: The results show significant decrease in cell numbers within 8 hours after HGE were inoculated, which the polystyrene surface was preincubated with 50 mg/ml HSA. But it did not prove to be the case from 10 hours to 24 hours after HGE were inoculated. There were no significant difference within 24 hours in cell numbers between cultured in KSFM with 50 mg/ml HSA and control. The cell numbers in cell growth curve of HGE in KSFM with and without 50 mg/ml HSA did not have significant difference. Conclusions: It was in the early stage that the cell attachment of HGE was inhibited with HSA preincubation on polystyrene.;