针刺与艾灸对慢性不可预见性温和应激抑郁大鼠前额皮质5-羟色胺系统的影响

目的:探讨针刺与艾灸对慢性不可预见性温和应激(CUMS)抑郁大鼠的疗效差异及其机制。方法:SD大鼠随机分为空白组、模型组、氟西汀组、针刺组、艾灸组,每组8只。采用CUMS复制抑郁大鼠模型。针刺组针刺"百会""大椎",艾灸组艾灸"百会""大椎",均每次20min,每日1次,共治疗3周。计算糖水消耗百分比,采用放射免疫法检测前额皮质5-羟色胺(5-HT)、5-羟吲哚乙酸(5-HIAA)、色氨酸(Trp)含量,放射性配体受体结合分析技术计算前额皮质高低亲和力5-HT1A受体的结合常数(K)、结合容量(R)。结果:模型组糖水消耗百分比较正常组显著下降(P0.01)。治疗3周后,各治疗组糖水消耗百分比较之模型组显著升高(P0.01)。模型组5-HT含量低于正常组(P0.01),氟西汀组较模型组升高(P0.05),艾灸组显著低于氟西汀组(P0.01)。模型组5-HIAA含量与正常组比较差异无统计学意义(P0.05),各治疗组均显著低于模型组(P0.01),艾灸组显著低于针刺组(P0.01)。5-HT/5-HIAA比值,模型组显著低于正常组(P0.01),各治疗组均显著高于模型组(P0.01,P0.05),艾灸组低于氟西汀组(P0.05)。模型组Trp含量显著高于正常组(P0.01),各治疗组则均显著低于模型组(P0.01)。与正常组比较,模型组K 1、R 2显著下降(P0.05),与模型组比较,各治疗组高低亲和力5-HT1A受体的结合常数与容量均有上升趋势(P0.05)。结论:针刺与艾灸对CUMS抑郁模型大鼠的疗效相当,调节5-HT系统及5-HT1A受体功能可能是针刺与艾灸抗抑郁的作用途径之一。

Effect of Acupuncture and Moxibustion Interventions on Ethological Changes and 5-HT/5-HIAA Levels in Prefrontal Cortex in Depression Rats

Objective To observe the effect of acupuncture and moxibustion interventions on ethology and 5-HT/5-HIAA levels in the frontal cortex tissue in depression rats,so as to reveal their biological mechanisms underlying improvement of depression.Methods A total of 40 male SD rats were randomized into normal control,depression model,medication(Fluoxetine),acupuncture and moxibustion groups,with 8rats in each group.The depression model was established by chronic unpredictable mild stress for 5weeks.The interventions including acupuncture or moxibustion stimulation of"Baihui"(GV 20)and"Dazhui"(GV14)for 20min/d and intragastric administration of Fluoxetine(1.8mg·kg-1·d-1)beginning after 2weeks of modeling for 3weeks.The percentages of sucrose consumption was counted,the contents of 5-HT,hydroxyindole acetic acid(5-HIAA),tryptophan(Trp)in the prefrontal cortex tissue were assayed by radioimmunoassay,and the relative binding affinities(constant and capacity)of 5-HT1 Areceptor in the prefrontal cortex were measured by radioligand binding assay and Scatchard plot analysis in a computer.Results In comparison with the normal control group,the sucrose consumption percentage on the 35 th day of modeling,the levels of cerebral cortical 5-HT,5-HT/5-HIAA and 5-HT1 Areceptor binding constant were significantly down-regulated in the model group(P<0.01,P<0.05),and the cortical Trp content was obviously up-regulated in the model group(P<0.01).Compared with the model group,the sucrose consumption percentage on the 35 th day of modeling,and cortical 5-HT/5-HIAA in the medication,acupuncture and moxibustion groups,cortical 5-HT content in the medication group were significantly increased(P<0.01,P<0.05),and cortical 5-HIAA and Trp contents were obviously down-regulated in the three treatment groups(P<0.01).No significant changes were found in the cortical 5-HT content after acupuncture and moxibustion,and in the high and lower affinity of 5-HT1 Areceptor binding constant and binding capacity after medication,acupuncture and moxibustion interventions(P>0.05).The effects of acupuncture and moxibustion interventions were comparable in increasing sucrose consumption percentage on the 35 th day and 5-HT/5-HIAA,and in down-regulating cortical Trp content(P>0.05).Conclusion Both acupuncture and moxibustion can increase sucrose consumption(anti-depression)in depression rats,which may be closely associated with their effects in up-regulating 5-HT/5-HIAA and down-regulating Trp content in the frontal cortex.