降钙素基因相关肽、P物质在电针缓解便秘型肠易激综合征中的作用

目的:观察电针对便秘型肠易激综合征大鼠(C-IBS)结肠组织中降钙素基因相关肽(CGRP)、P物质(SP)蛋白表达的影响,探讨针刺治疗C-IBS的可能机制。方法:Wistar大鼠随机分为正常对照组、模型组、电针大肠俞组、电针上巨虚组,每组8只。采用冰水灌胃法复制C-IBS模型。造模成功后电针治疗,每天1次,连续7d。观察各组大鼠粪便性状并进行粪便性状评分,同时检测粪便含水量;采用Western blot法检测大鼠结肠CGRP、SP蛋白表达量。结果:模型组粪便性状评分和粪便含水量明显低于正常对照组(P0.01),电针大肠俞组和电针上巨虚组大鼠粪便性状评分和粪便含水量均明显高于模型组(P0.01,P0.05)。模型组结肠中CGRP、SP蛋白的含量明显高于正常对照组(P0.01),电针大肠俞组和电针上巨虚组大鼠结肠中CGRP、SP蛋白含量均明显低于模型组(P0.01)。结论:CGRP、SP在电针缓解C-IBS内脏高敏感性中起重要作用。

Effect of Electroacupuncture Intervention on Constipation-predominant Irritable Bowel Syndrome and Colonic CGRP and SP Expression in Rats

Objective To observe the effect of electroacupuncture(EA)intervention on constipation-predominant irritable bowel syndrome(C-IBS)and colonic calcitonin gene-related peptide(CGRP)and substance P(SP)expression levels in IBS rats,so as to reveal its mechanism underlying improvement of IBS.Methods A total of 32 male Wistar rats were equally randomized into normal control,model,EA-Shangjuxu(ST 37)and EA-Dachangshu(BL 25)groups.The C-IBS model was established according to Peng's methods(2004).EA(2Hz/100 Hz,0.1-0.2mA)was applied to bilateral ST 37 and BL 25for30 min,once daily for 7days.The stool properties(hardness or softness,water content,etc.)were typed(1-7points)and the fecal water content was calculated by wet weight minus dry weight.The relative expression levels of CGRP and SP proteins in the colon tissues were detected by Western blot.Results Compared to the control group,the stool property score and stool water content were significantly lower in the model group(P<0.01).After EA intervention,both stool property scores and stool water contents were obviously higher in the EA-ST 37 and EA-BL 25 groups than in the model group(P<0.05,P<0.01).The expression levels of colonic CGRP and SP proteins were significantly higher in the model group than in the control group,and obviously down-regulated in the two EA intervention groups(P<0.01).No significant differences were found between the EA-ST 37 and EABL 25 groups in the stool property score and stool water content,and colonic CGRP and SP protein expression levels(P>0.05).Conclusion EA intervention can ameliorate the fecal property in C-IBS rats,which may be associated with its function in inhibiting the expression of colonic CGRP and SP proteins.