经皮穴位电刺激对多囊卵巢综合征大鼠激素及卵巢甾体激素代谢酶表达的影响

目的:观察经皮穴位电刺激对来曲唑诱导的多囊卵巢综合征(PCOS)大鼠血清性激素水平及卵巢甾体激素代谢酶表达的影响,探讨经皮穴位电刺激治疗PCOS的可能机制。方法:SD大鼠用随机数字表法分为正常组、模型组、药物组、经皮穴位电刺激组,每组10只。模型组、药物组、经皮穴位电刺激组大鼠用来曲唑灌胃诱导PCOS模型。经皮穴位电刺激组选取"关元""三阴交"进行电刺激干预,药物组给予克罗米芬1mg·kg-1·d-1灌胃,治疗7d。记录大鼠体质量、卵巢质量,HE染色观察卵巢形态结构,放射免疫法检测血清激素水平,免疫组化及Western blot法检测卵巢细胞色素P 450芳香化酶(P 450arom)和P 450 17-α羟化酶(P 450c17α)的表达。结果:模型组大鼠与正常组相比,体质量和卵巢质量均显著增加(P0.01),血清睾酮(T)、促黄体生成素(LH)水平及LH/促卵泡生成素(FSH)明显升高(P0.01),雌二醇(E2)水平明显降低(P0.01);经皮穴位电刺激组与模型组相比,体质量和卵巢质量显著降低(P0.01),血清T、LH水平及LH/FSH较模型组明显降低(P0.01,P0.05),卵巢形态结构接近正常。与正常组相比,模型组大鼠卵巢组织P 450c17α的表达明显增高(P0.01),P 450arom的表达明显降低(P0.01);经皮穴位电刺激组与模型组相比,P 450c17α的表达明显降低(P0.05),P 450arom的表达明显增高(P0.05)。结论:经皮穴位电刺激疗法可能通过调节PCOS模型大鼠卵巢甾体激素代谢酶P 450c17α和P 450arom的表达,从而调节性激素水平,改善PCOS模型大鼠生殖内分泌紊乱状态和卵巢发育状况。

Effect of Transcutaneuos Acupoint Electrostimulation on Serum Sex Hormone Levels and Expression of Ovarian Steroid Hormone Metabolic Enzymes in Polycystic Ovary Syndrome Rats

Objective To observe the effect of transcutaneuos acupoint electrostimulation(TAES)on ovarian serum sex hormone levels and ovarian follicle granular cell aromatase cytochrome P 450(P 450arom)protein and follicle theca cell cytochrome P 450 17α-hydroxylase/c 17-20 lyase cytochrome P 450(P 450c17α)protein expression in polycystic ovary syndrome(PCOS)rats,so as to explore its mechanisms underlying improvement of PCOS.Methods Forty SD rats were randomly divided into four groups:normal control,model,medication and TAES(10rats/group).The PCOS model was established by giving(gavage)the animals with letrozole solution(1.0mg/kg,once daily for 21 consecutive days).Rats of the medication group were treated with Clomiphene(1mg/kg)once daily for 7days,and those of the TAES group were treated with electrical stimulation(2Hz,3mA)of"Guanyuan"(CV 4)and"Sanyinjiao"(SP 6)areas for 30 min,once daily for 7consecutive days.The rats body weight and bilateral ovarian weight were detected,and the ovarian structure and follicular development degree were observed under light microscope after H.E.stain,and the serum testosterone(T),estradiol(E2),luteotrophic hormone(LH)and folliclestimulating hormone(FSH)contents were detected using radioimmunoassay.The expression of ovarian P 450arom(for production of estrogen)protein and P 450c17α(for production of androgen)protein was detected by using immunohistochemical stain and Western blot,respectively.Results The body weight,bilateral ovary weight,serum T and LH contents,and ratio of LH/FSH,and ovarian P 450c17αimmunoactivity and protein expression levels in the model group were all significantly increased compared with the normal control group(P<0.01),and the levels of serum E2 and ovarian P 450 arom immunoactivity and protein expression were significantly decreased after modeling(P<0.01).Following the treatment,the increased body weight,ovary weight,serum T and LH contents,ratio of LH/FSH,and ovarian P 450c17αimmunoactivity and protein expression levels,and the decreased ovarian P 450 arom immunoactivity and protein expression levels were reversed in the TAES group(P<0.01,P<0.05)rather than in the medication group,except serum T and ratio of LH/FSH in the medication group.No significant differences were found between the medication and TAES groups in the serum T and ratio of LH/FSH(P>0.05).In addition,the increased vesicular follicle number,the decreased corepus luteum number and the thickness of granular cell layer were markedly improved in the TAES group.Conclusion TAES intervention can reduce both body weight and ovarian weight and regulate the levels of serum sex hormones and ovarian P 450c17αand P 450 arom protein expression levels in PCOS rats,which may contribute to its effect in improving PCOS.