神经降压素介导的脑-肠轴在电针治疗功能性消化不良大鼠中的作用

目的:探讨神经降压素(neurotensin,NT)介导的脑-肠轴在电针治疗功能性消化不良(FD)大鼠中的作用。方法:SD大鼠48只按照随机数字表法分为空白组、模型组、电针组,每组16只。采用夹尾刺激法配合隔日进食制备FD大鼠模型。电针组电针右侧"足三里"和"太冲"穴,每次30min,每日1次,共14d。检测胃排空率和小肠推进率,运用ELISA法检测血浆中NT含量,应用免疫组织化学法检测下丘脑、胃窦及回肠组织中NT的表达。结果:模型组较空白组大鼠胃排空率明显降低(P0.01),小肠推进率显著下降(P0.01);电针组较模型组胃排空率增加,小肠推进率升高(P0.05)。模型组血浆NT含量较空白组增加(P0.05),电针组较模型组明显减少(P0.05)。模型组下丘脑、胃窦及回肠组织中NT表达较空白组升高(P0.05),电针组较模型组降低(P0.05)。结论:电针可明显降低FD大鼠下丘脑、胃窦、回肠及血浆中NT的表达,通过中枢及外周两种途径介导脑-肠轴作用,加快胃排空及小肠推进,改善胃肠动力障碍,该作用可能是电针治疗FD的重要机制之一。

Involvement of Neurotensin-mediated Brain-gut Axis in Electroacupuncture Intervention Induced Improvement of Functional Dyspepsia in Rats

Objective To observe The effect of electroacupuncture(EA)stimulation of"Zusanli"(ST 36)and "Taichong"(LR 3)on intestinal motor and neurotensin(NT)levels in the plasma,hypothalamus,and gastro-antrum tissues in functional dyspepsia(FD)rats so as to reveal its mechanisms underlying improvement of FD.Methods Forty-eight SD rats were randomly divided into control,model and EA groups,with 16 rats in each group.The FD model was established by clamping the rats' tails and alternate day's feeding according to the related references.EA(2Hz/100 Hz,2mA)was applied to unilateral ST 36 and LR 3for 30 min,once daily for 14 days.Rats of the control group were only restricted.The gastric emptying rate and propulsive rate of the small intestine were detected.The content of NT in the plasma was assayed using ELISA,and the immunoactivity levels of NT in the hypothalamus,gastric antrum mucous membrane and ileum tissues were detected using immunohistochemistry.Results Compared with the control group,the gastric emptying rate and propulsive rate of the small intestine were considerably lowered in the model group(P<0.01),and the content and immunoactivity levels of NT in the plasma,hypothalamus,mucous membrane of the gastric antrum and ileum tissues were significantly increased(P<0.05).After EA intervention,the decreased gastric emptying rate and intestinal propulsive rate,as well as the increased NT content and immunoactivity levels of plasma,hypothalamus,gastric antrum and ileum were reversed(P<0.05).Conclusion EA intervention can obviously promote gastrointestinal motor in FD rats,which may be related to its function in down-regulating NT levels in the plasma,hypothalamus,gastric antrum and ileum.It suggests an involvement of NT in the brain-gut axis in EA-induced improvement of FD.