首页 | 本学科首页   官方微博 | 高级检索  
     


Saccharomyces cerevisiae Ats1p interacts with Nap1p,a cytoplasmic protein that controls bud morphogenesis
Authors:Christina?M.?Shields,Rachel?Taylor,Tara?Nazarenus,Joseph?Cheatle,Ann?Hou,Audrey?Tapprich,Alexis?Haifley,Audrey?L.?Atkin  author-information"  >  author-information__contact u-icon-before"  >  mailto:aatkin@biocomp.unl.edu"   title="  aatkin@biocomp.unl.edu"   itemprop="  email"   data-track="  click"   data-track-action="  Email author"   data-track-label="  "  >Email author
Affiliation:(1) School of Biological Sciences, University of Nebraska—Lincoln, Lincoln, NE 68588-0666, USA
Abstract: Saccharomyces cerevisiae ATS1 (agr-tubulin suppressor 1) was originally identified as a high-copy suppressor of class two agr-tubulin mutations and was proposed to have a regulatory role in coordinating the microtubule state with the cell cycle. Here, we show that Ats1p interacts with Nap1p, a cytoplasmic protein that regulates the activity of the Cdc28p/Clb2p complex. Loss of Nap1p results in a delayed switch from polar to isotropic bud growth. The delayed switch results in elongated buds. Nap1p and Ats1p interact in two-hybrid and co-immunoprecipitation assays. Both nap1Delta and ats1Delta cells have a Clb2p-dependent elongated bud morphology. Deletion of ATS1 partially suppresses the elongated bud morphology and benomyl resistance of nap1Delta mutants. Our results suggest Ats1p might regulate coordination of the microtubule state with the cell cycle through an interaction with Nap1p.Communicated by S. Hohmann
Keywords:Ats1p  Nap1p  Clb2p  Cell cycle  Morphogenesis checkpoint
本文献已被 PubMed SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号