SGT蛋白在小鼠脑发育过程中的表达

目的通过研究SGT蛋白在小鼠脑发育过程中的表达来揭示该蛋白在小鼠脑发育阶段中的重要性。方法分别运用定量RT-PCR以及Western blot实验方法来检测小鼠全脑中SGT核酸和蛋白的表达情况。用Westernblot实验方法检测SGT蛋白在海马,皮层,纹状体和小脑中的表达模式;运用免疫组化方法来检测SGT蛋白在小鼠脑分区中的亚细胞定位。结果在小鼠全脑中,SGT的核酸和蛋白水平均表现为出生早期的高表达,到了成年期,表达水平下降。SGT蛋白在海马,皮层,纹状体和小脑中的表达模式和在全脑中的类似。免疫组化结果显示,在出生后14天(P14)的小鼠脑切片中,SGT蛋白在海马的CA区域以及皮层和纹状体都有强的阳性表达。在小脑中,SGT蛋白主要分布在浦肯野细胞层。在同样的检测条件下,SGT蛋白在成年期小鼠的脑切片中没有明显的阳性表达。结论我们的研究首次揭示了SGT蛋白在小鼠脑发育过程中的表达,为进一步揭示SGT在中枢神经系统中的功能提供了一个参考依据。

Expression of small glutamine-rich TPR-containing protein in the developing mouse brain

Purpose The aim of this study was to reveal the role of SGT,the small glutamine-rich tetratricopeptide repeat(TPR)-containing protein,in the developing mouse brain through examining the expression profile of SGT during the development stage.Methods In this study,quantitative RT-RCR and Western blot were applied to investigate the expression of SGT mRNA and protein in the mouse whole brain.Western bolt was also used to detect the expression of SGT in the hippocampus,cerebral cortex,striatum and cerebellum.Immunohistochemical analysis on postnatal and adult mouse brain was performed to examine the subcellular localization of SGT.Results Our data showed that the levels of SGT mRNA and protein in the mouse whole brain were both high during the postnatal stage and declined in the adult.Regional expression of SGT protein in the hippocampus,cerebral cortex,striatum and cerebellum showed a similar expression profile.Immunohistochemical analysis found that in the P14 mouse brain,SGT was abundant in all the CA regions of hippocampus as well as most regions of cerebral cortex and striatum.In the cerebellum,SGT was mainly distributed in Purkinje cells.In the sections of the adult mouse brain,faint expression was observed in the regions mentioned above.Conclusions Our findings firstly exhibit the expression pattern of SGT in the mouse brain development,which might shed new light on further functional analysis of SGT in the central nervous system.