| 乌头类生物碱对ras基因表达影响及其抗肿瘤分子机制研究 |
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| 引用本文： | 饶朝龙,彭成.乌头类生物碱对ras基因表达影响及其抗肿瘤分子机制研究[J].现代预防医学,2010,37(6). |
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| 作者姓名： | 饶朝龙 彭成 |
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| 作者单位： | 成都中医药大学,成都,610075 |
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| 基金项目： | 国家重点基础研究发展规划(973计划)，国家自然科学基金重点项目 |
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| 摘 要： | 目的]研究乌头类生物碱对细胞增殖周期和ras基因表达的影响,探讨乌头类中药抗肿瘤作用的分子机制。方法]体外培养大鼠视网膜神经细胞,分别以0.5%的乌头碱、新乌头碱和次乌头碱作用细胞5min后,流式细胞仪检测。结果]乌头碱、新乌头碱和次乌头碱作用下,细胞增殖活动被抑制,ras基因表达量显著下降。结论]ras基因及Ras/Raf/MEK/MAPK信号级联通路可能是乌头类中药抗肿瘤作用的重要靶点。
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| 关 键 词： | 乌头类生物碱 ras基因 Ras/Raf/MEK/MAPK信号通路 |
STUDY ON THE EFFECT OF ACONITIC ALKALOID ON RAS GENE EXPRESSION AND ITS MOLECULAR MECHANISM OF ANTITUMOR ACTIVITY |
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| RAO Chao-long,PENG Cheng.STUDY ON THE EFFECT OF ACONITIC ALKALOID ON RAS GENE EXPRESSION AND ITS MOLECULAR MECHANISM OF ANTITUMOR ACTIVITY[J].Modern Preventive Medicine,2010,37(6). |
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| Authors: | RAO Chao-long PENG Cheng |
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| Institution: | RAO Chao-long,PENG Cheng. (Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China) |
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| Abstract: | Objective] To study the effect of aconitic alkaloid on cell cycle and ras gene expression, and explore its molecular mechanism of anti-tumor activity. Methods] Rat retina neuron was cultivated in vitro, and treated with aconitine, mesaconitine and hypaconitine for 5 minutes respectively, then determined with Flow Cytometer. Results] With the effect of aconitine, meaconitine and hypaconitine, cell proliferation decreased significantly, and amount of ras gene expression decreased significantly. Conclusion... |
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| Keywords: | Aconitic alkaloid Ras gene Ras/Raf/MEK/MAPK signal pathway |
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