丁型肝炎病人肝组织HDVRNA与 HBVDNA的表达及关系

目的 探讨丁型肝炎病人肝组织中ＨＤＡｇ、ＨＤＶＲＮＡ与ＨＢＶＤＮＡＧ表达及关系。方法 应用免疫组化和原位杂交技术检测了７９全丁型肝炎病人肝组织ＨＤＡｇ、ＨＤＶＲＡＮ～ＨＢＶＤＮＡ表达，以５２例型肝炎病人肝组织作对照。结果 丁型肝炎ＨＢＶＤＮＡ检出率（２７％）低于乙型肝炎（４４％）（Ｐ〈０．０５）。在坏死灶边缘肝细胞和气球样变肝细胞浆内有大量的ＨＤＶＲＮＡ蓄积或ＨＤＡｇ呈强型强表达，ＨＤＶＲＮＡ表达

Study on the expression and relationship between HDVRNA and HBVDNA in liver tissue of the patients with hepatitis D

ObjectiveTo explore the expression and relationship of hepatitis D virus antigen (HDAg)/hepatitis delta virus ribonucleic acid(HDVRNA) and hepatitis B virus deoxyribonucleic acid(HBVDNA) in liver tissue of the patients with hepatitis D(HD)Methods HDAg,HDVRNA and HBVDNA in paraffinembedded liver tissue from 79 liver biopsy or autopsy samples from the patients with HD were studied with immunohistochemical techniques and hybridization in situ,meanwhile,compared with the expression of HBVDNA in liver tissues of the patients with hepatitis B(HB)ResultsIt showed that the detection rate of HBVDNA in liver specimens of HD(27%,21/79) was lower than that of hepatitis B(44%,23/52)(P<005).Strong expression of HDAg and HDVRNA were shown in the bollooning degeneration hepatocytes and hepatocyts near piecemeal necrosis region.HDAg expression was significently negative association with HBVDNA expression(P<005).ConclusionHDV infection can inhibit replication of HBVDNA,espcially at strong expressiong of HDAg or HDVRNA. Besides interactions between HBV and HDV,it is possible that direct cytotoxicity of HDV may play an important pathogenetic role in pathogenetic mechanisms of HDV.