泡球蚴组织的体外培养及在药物筛选中的应用

目的 建立泡球蚴组织的体外培养模型，对4种药物的体外抗泡球蚴疗效进行初步筛选。方法 泡球蚴组织体外培养9周后，分为7组：阿苯达唑组、汉防己甲素组、己酮可可碱组、苦豆碱组、氧氟沙星组、未用药组和二甲基亚砜组。在加药24h和18d后，分别取样固定并在电子显微镜下观察超微结构。实验期间对囊泡计数。实验结束后，将培养物接种BALB／c小鼠进行活力测试。结果 汉防己甲素和己酮可可碱在体外能抑制泡球蚴的生长（P〈0．005），氧氟沙星对泡球蚴没有作用（P〉0．005），苦豆碱能促进泡球蚴生长。接种各药物组培养物的BALB／c小鼠饲养80d后处死并解剖，发现所有小鼠的腹腔中均有泡球蚴生长。结论 汉防己甲素和己酮可可碱在体外对泡球蚴有一定的抑制作用，氧氟沙星和苦豆碱对寄生虫无治疗作用。活力测试提示所选药物只能抑制寄生虫生长而无杀灭作用。

In vitro culture of Echinococcus multilocularis metacestodes to screen the efficiency of drugs

Objective Echinococcus multilocularis metacestodes were cultured in vitro successfully and the efficiency of tetrandrine,pentoxifylline,ofloxacin and aloperine on E.multilocularis metacestodes was screened.Methods After culturing for 9 weeks,the metacestode tissues were divided into 7 groups,albendazole,tetrandrine,pentoxifylline,ofloxacin and aloperine were separately added in each culture flask.The appropriate controls included(i) a culture containing an equal amount of DMSO and(ii) a culture in culture medium alone.During the experiment,vesicles were counted every day.24 h and 18 d post-treatment,the parasite tissues were fixed and observed under electron microscope.In order to determine the viability of metacestodes,female BALB/c mice were infected by intraperitoneal injection with vesicles.Results Tetrandrine and pentoxifylline can inhibit the growth of metacestodes in vitro(P<0.005),there were not effect on metacestodes of ofloxacin(P>0.005) and aloperine could enhance the growth of metacestodes in vitro(P<0.005).Metacestode tissues were developed in all animals at 80 days after infection.Conclusion Tetrandrine and pentoxifylline could inhibit the growth of E.multilocularis metacestodes in vitro,but ofloxacin and aloperine had no effect on the parasite.The proliferation of E.multilocularis in mice suggests that the tetrandrine and pentoxifylline are the parasitostatic,but without parasitocidal effects on larval stage of E.multilocularis.