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| 熊果酸对人胃癌细胞株MGC-803的抑制作用及其机制 | |
| 引用本文： | 王琼,陈建军,向谨逸.熊果酸对人胃癌细胞株MGC-803的抑制作用及其机制[J].山东医药,2007,47(27):30-31. |
| 作者姓名： | 王琼 陈建军 向谨逸 |
| 作者单位： | 1. 湖北省新华医院,湖北武汉,430015 2. 崇阳县人民医院 3. 华中科技大学同济医学院 |
| 摘 要： | 目的观察熊果酸（UA）对胃癌细胞株MGC-803的抑制增殖作用。方法培养胃癌细胞株MGC-803，以MTT比色法及生长曲线测定UA对MGC-803抑制增殖的作用。免疫印记法测定p-ERK1/2、CyclinD1、p21^waf/tip。表达。结果MTT实验及生长曲线均显示UA明显抑制MGC-803细胞增殖，免疫印记法显示UA抑制pERK1/2、CyclinD1表达，同时上调p21^waf/vip。表达。结论熊果酸可以抑制MGC-803增殖，机制与影响p-ERK1／2及下游CyclinD1、p21^waf/cipl表达有关。 |
| 关 键 词： | 熊果酸 胃肿瘤 细胞外信号调节激酶 增殖 |
| 文章编号： | 1002-266X（2007）27-0030-02 |
| 修稿时间： | 2007-05-24 |
Effect of Ursolic acid on the proliferation of human gastric carcinoma MGC-803 cell line and it's mechanism |
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| WANG Qiong,CHEN Jian-jun,XIANG Jin-yi.Effect of Ursolic acid on the proliferation of human gastric carcinoma MGC-803 cell line and it''''s mechanism[J].Shandong Medical Journal,2007,47(27):30-31. | |
| Authors: | WANG Qiong CHEN Jian-jun XIANG Jin-yi |
| Institution: | 1 Xinhua Hospital of Hubei Province, Wuhan 430015, P. R. China |
| Abstract: | Objective ] To observe the effects of Ursolic acid on the proliferation of human gastric carcinoma MGC- 803 cell line. Methods] The effect of Ursolic acid on the proliferation of MGC-803 cells wat- observed by MTT assay and growth curve. The expression of p-ERK1/2, Cyclin D1, p21^waf/cipl was analyzed by Western blot. Results ] Ursolic acid could depress the proliferation of MGC-803 cell line. The expressions of p-ERK1/2 and Cyclin D1 were decreased, while p21^waf/cipl expression was elevated by different concentrations of Ursolic acid, Conclusion ] Ursolic acid can depress the proliferation of MGC-803 cell line with inhibiting ERK signal transduction, |
| Keywords: | Ursolic acid gastric carcinoma extraceUular signal-regulated kinase proliferation |
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