脾切除肝硬化门脉高压大鼠血浆TNF-α质量浓度的变化

为研究脾切除术、脾动脉结扎术对肝硬化门脉高压症 (PHT)大鼠血浆肿瘤坏死因子 (TNF α)的影响 ,研究PHT时TNF α与一氧化氮合酶 (NOS)间的关系 ,建成PHT大鼠模型 ,用放射免疫分析法动态检测TNF α ,根据吸光度的大小测定血浆NOS活性 ,并观察术后 4周内各组的病死率。发现 :PHT大鼠血浆TNF α质量浓度较正常对照组明显增高 (P <0 .0 1 ) ;PHT脾切除组术后 3d、7d、1 4d血浆TNF α质量浓度与实验对照组比较有显著性差异 (P <0 .0 1 ) ;脾动脉结扎组TNF α的质量浓度虽比实验对照组升高 ,但无显著性差异。实验对照组血浆NOS活性明显高于正常对照组 (P <0 .0 1 ) ,且与TNF α显著正相关 (P <0 .0 1 ,r =0 .946) ;脾动脉结扎组NOS活性显著低于实验对照组及脾切除组 (P <0 .0 1 )。PHT时血浆TNF α的质量浓度显著增高 ,提示 :TNF α可能在PHT形成中起重要作用。与脾动脉结扎组相比较 ,脾切除组血浆TNF α的质量浓度显著增高 ,血浆NOS活性以及病死率亦显著增高 ,提示 :脾动脉结扎术后脾脏仍可发挥一定作用 ,PHT时应保留脾脏。

Effect of Tumor Necrosis Factor on Venous Plasma of Portal Hypertension Rat Perserved Splen

The aim was to study the effect of portal hypertension (PHT) on tumor necrosis factor (TNF-α) in venous plasma, the influence of splenectomy and splenic artery ligation on TNF-α levels in venous plasma in model rats and to investigate the relation between TNF-α and NOS levels in venous plasma in PHT rats. Healthy Wistar rats were randomized into four groups: The latter three groups were used to established PHT model. TNF-α levels in venous plasma were dynamically measured by radioimmunoassay (RIA) at points after the splenectomy and SAL; NOS levels in venous plasma were determined 14 d after operation. The mortality between SLC group and SAL group were compared 4 weeks after operation. Light microscope were applied to observe the pathological change. PHT TNF-αlevels in venous plasma were significantly higher than NCG group (P<0.01). SLC group TNF-αin plasma increased significantly, compared with MCG group(P<0.01), at points (3 d, 7 d, and 14 d) after splenectomy. SAL group TNF-α levels in venous plasma were slightly higher than MCG group (P> 0.05), and were significantly lower than SLC group (P< 0.01) at points (3 d, 7 d, 14 d) after SAL. MCG group NOS levels in venous plasma were significantly elevated(P<0.01), compared with NCG group, there was a positive correlation(r=0.946, P<0.01) between TNF-α levels and NOS levels in venous plasma, while SAL group NOS levels were significantly reduced (P< 0.01), compared with MCG group and SLC group respectively. The morbidity in SLC group was remarkably higher than that of SAL group (P< 0.05). In PHT, TNF-α levels in venous plasma significantly increase, possibly play an important role in the course of PHT. TNF-αlevels in venous plasma significantly increase after splenectomy, while the morbidity also elevate remarkably. Compared with PHT model rats, SAL group TNF-α levels and the morbidity don't increas significantly, and prognosis can be improved.