| 人角膜细胞的原代培养实验研究 |
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| 引用本文： | 谢立信,董晓光.人角膜细胞的原代培养实验研究[J].潍坊医学院学报,1990,12(1):10-13. |
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| 作者姓名： | 谢立信 董晓光 |
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| 作者单位： | 潍坊医学院眼科角膜病研究所，潍坊医学院眼科角膜病研究所，潍坊医学院眼科角膜病研究所，潍坊医学院眼科角膜病研究所，潍坊医学院眼科角膜病研究所 |
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| 摘 要： | 报告了人角膜细胞的体外培养技术,成功地建立了人角膜上皮、基质和内皮细胞的原代纯培养且形成良好的细胞单层。并对培养的三种细胞的形态进行了描述,可用于对角膜组织细胞的体外实验研究,并讨论了在该项工作中获得成功的经验和注意事项。
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| 关 键 词： | 人 角膜细胞 体外培养 |
Experimental Studies on Primary Gulture of Human Corneal Cells |
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| Xie Lixin,et al.Experimental Studies on Primary Gulture of Human Corneal Cells[J].Journal of Weifang Medical College,1990,12(1):10-13. |
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| Authors: | Xie Lixin |
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| Institution: | Corneal Research Laboratories of Weifang Medical College |
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| Abstract: | This paper reports the culture techniques for human corneal cells. Using this method, we have successfully established pure primary culture of three types of cells (epithelium, keratocyte, endothelium), every of which can form an adherent monolayer and become confluent. This paper also describes the morphology of each cell type in vitro. The culture of three types of cells allows their struxtur and function to be studied in vitro. The successful experience and notes are also discussed. |
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| Keywords: | human corneal cell in vitro culture |
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