| 高效液相色谱法测定中药退黄外洗液中大黄酸、大黄素、大黄酚的含量 |
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| 引用本文： | 宾驰,颜栋林.高效液相色谱法测定中药退黄外洗液中大黄酸、大黄素、大黄酚的含量[J].临床合理用药杂志,2011(17):42-44. |
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| 作者姓名： | 宾驰 颜栋林 |
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| 作者单位： | [1]广西壮族自治区浦北县人民医院,535300 [2]南宁市食品药品检验所,530001 |
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| 摘 要： | 目的建立中药退黄外洗液中大黄酸、大黄素、大黄酚的含量测定方法。方法采用高效液相色谱(HPLC)法测定大黄酸、大黄素、大黄酚的含量,色谱柱:Phenomenex Gemini5μm C18110A4.6×250.0mm5micron;流动相:甲醇-0.1%磷酸溶液(85∶15),流速:1.0ml/min,检测波长:254nm,柱温:35℃。结果大黄酸在1.6576～33.1520μg/ml、大黄素在1.4976～29.9520μg/ml、大黄酚在1.7040～34.0800μg/ml范围内线性关系良好(r=1)。大黄酸平均回收率为99.07%,RSD为0.85%(n=5);大黄素为99.14%,RSD为1.08%(n=5);大黄酚为99.94%,RSD为0.77%(n=5);阴性对照无干扰。结论该方法操作简便、稳定、专属性强,可作为该制剂的质量控制方法。
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| 关 键 词： | 高效液相色谱法 中药退黄外洗液 大黄酸 大黄素 大黄酚 |
Determination of rhein,emodin and chrysophanol in Chinese Medicine Tuihuang outside lotion by HPLC |
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| BIN Chi＊,YAN Dong-lin.Determination of rhein,emodin and chrysophanol in Chinese Medicine Tuihuang outside lotion by HPLC[J].Chinese Journal of Clinical Rational Drug Use,2011(17):42-44. |
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| Authors: | BIN Chi＊ YAN Dong-lin |
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| Institution: | .＊The People＇s Hospital of Pubei,Pubei,Guangxi Zhuang Autonomous Region 535300,China |
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| Abstract: | Objective To establish a method for determination of rhein,emodin and chrysophanol in Chinese Medicine Tuihuang outside lotion.Methods Determinedf rhein,emodin,chrysophanol by HPLC,column：Phenomenex Gemini 5μm C18 110A 4.6×250.0mm 5micron;Mobile phase：Methanol-0.1% phosphoric acid（85：15）,flow rate：1.0ml/min,detection wavelength：254nm,column temperature：35℃.Results Rhein at 1.6576～33.1520μg/ml,emodin at 1.4976～29.9520μg/ml,chrysophanol at 1.7040～34.0800μg/ml range,had a good linear relationship（r=1）.The average recovery rhein was 99.07%,RSD=0.85%（n=5）;Emodin was 99.14%,RSD was 1.08%（n=5）;Chrysophanol was 99.94%,RSD was 0.77%（n=5）;The negative sample without interference.Conclusion This method is simple,stable,specific,and can be used for the quality control methods. |
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| Keywords: | HPLC Chinese Medicine Tuihuang outside lotion Rhein Emodin Chrysophanol |
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