Poly(L-lactic acid)/polyethylenimine nanoparticles as plasmid DNA carriers |
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Authors: | Yu-Mi Park Boo-Ahn Shin In-Joon Oh |
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Institution: | (1) College of Medicine, Chonnam National University, Hag-dong, Dong-gu, Gwangju, 501-746, Korea;(2) College of Pharmacy and Research Institute of Drug Development, and Nanotechnology Research Center, Chonnam National University, Yongbong-dong, Bug-gu, Gwangju, 500-757, Korea |
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Abstract: | Non-viral vectors such as liposomes, polycations, and nanoparticles have been used as gene delivery systems. In this study,
we prepared and characterized biodegradable poly(L-lactic acid) (PLA)/polyethylenimine (PEI) nanoparticles as gene carriers.
pCMV/β-gal and pEGFP-C1 were utilized as model plasmid DNAs (pDNA). Nanoparticles were prepared using a double emulsion-solvent
evaporation technique, and their pDNA binding capacity was assessed by agarose gel electrophoresis. Transfection was studied
in HEK 293 and HeLa cell lines, and the transfection efficiencies were determined by β-galactosidase assay or flow cytometry.
Three kinds of PLA/PEI systems were studied by varying the molecular weight of PEI. The PLA/PEI 25K system had a higher transfection
efficiency than the PLA/PEI 0.8K or PLA/PEI 750K systems. The transfection efficiency was found to be dependent on the ratio
of PLA/PEI nanoparticles to pDNA with an optimum ratio of 60:1 (w/w). The cytotoxicity was dependent on the quantity of PLA/PEI
nanoparticles used, but it was comparable to that of commercial Lipofectin™. These results demonstrate the potential of PLA/PEI
nanoparticles as gene carriers. |
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Keywords: | Nanoparticles Poly(L-lactic acid) Polyethylenimine Transfection Non-viral gene delivery |
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