| 降落PCR扩增人类常染色体STR D15S128 |
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| 引用本文： | 张艳萍,郭大玮,马莉莉.降落PCR扩增人类常染色体STR D15S128[J].中国现代医生,2010,48(2):38-39. |
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| 作者姓名： | 张艳萍 郭大玮 马莉莉 |
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| 作者单位： | 山西医科大学法医学院,山西太原,030001 |
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| 摘 要： | 目的优化PCR程序,尝试用降落PCR扩增人类常染色体STRD15S128。方法用普通PCR和降落PCR扩增人类常染色体STRD15S128。结果普通PCR扩增产物有非特异带,降落PCR无非特异带。结论降落PCR简化了普通PCR中摸索最适退火温度的过程,可以一步找到人类常染色体STRD15S128的退火温度,是一种高效率的PCR方法。
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| 关 键 词： | D15S128 降落PCR 退火温度 PCR特异性 |
Touchdown PCR for Amplification of Human Autosomal STR D15S128 |
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| Authors: | ZHANG Yanping GUO Dawei MA Lili |
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| Institution: | ZHANG Yanping GUO Dawei MA Lili College of Forensic Medicine of Shanxi Medical University,Taiyuan 030001,China |
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| Abstract: | Objective To optimize PCR program and try to amplify human autosomal STR D15S128 by touchdown PCR(TD-PCR).Methods We used both common PCR and TD-PCR to amplify human autosomal STR D15S128. Results Ordinary amplification showed non-specificity band and TD-PCR showed no non-specificity band. Conclusion Compared with ordinary PCR,TD-PCR is simple and effective. |
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| Keywords: | D15S128 |
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