| 大黄素对肝癌细胞SMMC-7721抑制作用及P53、C-myc蛋白的表达 |
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| 引用本文： | 贺学强,林鸿,郑宝轩,严祥. 大黄素对肝癌细胞SMMC-7721抑制作用及P53、C-myc蛋白的表达[J]. 中国中医药信息杂志, 2005, 12(1): 21-22 |
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| 作者姓名： | 贺学强 林鸿 郑宝轩 严祥 |
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| 作者单位： | 1. 中国人民解放军第181中心医院,广西,桂林,541002
2. 兰州医学院第一附属医院,甘肃,兰州,730000 |
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| 摘 要： | 目的 研究大黄素对肝癌细胞 SMMC-7721 的生长抑制作用和 P53、C-myc 蛋白的表达。方法 应用 MTT法观察大黄素、5-FU 对 SMMC-7721 的生长抑制作用,免疫组化法染色(SP 法)测定细胞凋亡时 P53、C-myc 蛋白的表达情况。结果 大黄素对肝癌细胞 SMMC-7721 有明显的生长抑制作用,其 IC50为 21.6 mol/L。大黄素对 SMMC-7721的抑制作用优于 5-FU(P<0.05);大黄素作用于细胞后,P53、C-myc 蛋白均有一定程度下调,而 C-myc 下调较 P53明显。结论 大黄素对 SMMC-7721 有明显的生长抑制作用,作用于 SMMC-7721 后 P53、C-myc 蛋白的表达均有下调。
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| 关 键 词： | 大黄素 肝细胞癌 P53 C-myc |
| 文章编号： | 1005-5304(2005)01-0021-02 |
| 修稿时间： | 2003-06-06 |
Effects of Emodin on Hepatocelluler Cancer Cells in Gro wthinhibiting and Expression of P53 and C-myc Protein |
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| HE Xue-qiang,LIN Hong,ZHENG Bao-xuan,et al. Effects of Emodin on Hepatocelluler Cancer Cells in Gro wthinhibiting and Expression of P53 and C-myc Protein[J]. Chinese Journal of Information on Traditional Chinese Medicine, 2005, 12(1): 21-22 |
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| Authors: | HE Xue-qiang LIN Hong ZHENG Bao-xuan et al |
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| Abstract: | Objective To study the effects of emodin on hepatocelluler cancer cells in growthinhibiting and expression of P53 and C-myc protein. Methods The growthinhibiting of hepatocelluler cancer cells in vitro were measured by MTT assy. The expression of P53 and C-myc protein in apoptosis were checked by immumnohistochemical staining. Results Emodin had remarkable effect of growthinhibiting on hepatocelluler cancer cells. IC50 of emodin SMMC-7721 were 21.65 mol/L. The effects of growthinhibiting on hepatocelluler cancer cells by emodin is better than 5-FU. Conclusions Emodin have obvious effects of growthinhibiting on hepatoccelluler cancer cells. P53 and C-myc protein have both fall after hepatocelluler cancer cells expose to emodin. |
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| Keywords: | emodin hepatocelluler cancer cells P53 C-myc |
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