宫颈液基薄层脱落细胞联合HPV及p16检测在宫颈癌早期筛查中的临床意义 |
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引用本文: | 陆晓青,;陈洁瑛,;舒青青,;朱晓萍,;刘素贞.宫颈液基薄层脱落细胞联合HPV及p16检测在宫颈癌早期筛查中的临床意义[J].浙江医学,2014(13):1147-1149. |
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作者姓名: | 陆晓青 ;陈洁瑛 ;舒青青 ;朱晓萍 ;刘素贞 |
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作者单位: | [1]衢州市妇幼保健院病理科, 324000; [2]衢州市妇幼保健院妇科, 324000; [3] 衢州市职业技术学院, 324000; |
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基金项目: | 衢州市科技局科研立项课题(20121092) |
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摘 要: | 目的:探讨薄层液基脱落细胞检查(TCT)、高危型乳头状瘤病毒(HPV)DNA检测及脱落细胞p16蛋白免疫细胞化学检测在宫颈癌早期筛查中的应用价值。方法对TCT、HC2- HPV- DNA检测至少一项异常的815例患者,利用剩余细胞学标本进行p16免疫组化检测,同时行阴道镜及病理组织学检查进行对照。结果 HC2- HPV- DNA的阳性率和宫颈脱落细胞中p16表达阳性率随着宫颈病变严重程度的增加而明显升高(P<0.01)。HC2- HPV- DNA+TCT检测提高了宫颈癌及癌前病变的检出率,p16免疫细胞化学检查则提高了检测的特异度。结论在TCT和HC2- HPV- DNA检测的基础上,联合应用脱落细胞p16免疫细胞化学染色,可提高宫颈癌早期筛查的准确性。
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关 键 词: | 薄层液基脱落细胞检查 人乳头状瘤病毒 p16 宫颈肿瘤 宫颈上皮内瘤变 |
Thin cytology test and HPV DNA detection combined with p16 immunocytochemistry in screening of early cervical cancer |
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Institution: | LU Xiao- qing, CHEN Jieying, SHU Qingqing, et al.( Department of Pathology, Quzhou Institute of Maternal and Child Health Care,Quzhou 324000, China) |
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Abstract: | Objective To evaluate the application of thin cytology test(TCT) and Hybrid Capture 2- high risk HPV- DNA test (HC2- HPV- DNA) combined with p16 immunocytochemical staining in screening of early cervical cancer. Methods Total 815 women underwent TCT and HC2- HPV- DNA testing, the specimens with at least one abnormal finding were subject to p16 im-munocytochemical examination, and biopsy under colposcopy was also performed for these patients. Results The positive rate of both HC2- HPV- DNA test and p16 expression increased with the degree of cervical lesions(P〈0.01). Simultaneous detection of TCT and HC2- HPV- DNA increased the detection rate of cervical cancers and cancer precursor lesions. Incorporation of these two tests with p16 Immunocytochemical staining improved the specificity of detection. Conclusion TCT and HC2- HPV- DNA detec-tion combined with immunocytochemical staining for p16 can improve the specificity of cervical cancer screening. |
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Keywords: | TCT Human papillary virus p16 Cervical tumor Cervical intraepithelial neoplasm |
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