抑制TUBA1C的过表达在卵巢癌细胞株CAOV3、SKOV3细胞增殖侵袭中的作用机制探讨

目的：探讨抑制α-微管蛋白特异性1c链（TUBA1C）过表达对卵巢癌细胞株CAOV3、SKOV3细胞增殖、侵袭的影响，初步阐明TUBA1C在上皮性卵巢癌中的作用机制。方法通过脂质体介导抑制TUBA1C基因的过表达质粒转染CAOV3、SKOV3细胞，CAOV3分为3组：CAOV3实验组、CAOV3空载体转染组和CAOV3空白对照组；SKOV3分为3组：SKOV3实验组、SKOV3空载体转染组和SKOV3空白对照组。采用RT- PCR法检测TUBA1C表达；CCK-8法及Transwel 小室体外侵袭实验评价TUBA1C基因抑制后对卵巢癌细胞增殖、侵袭的影响。结果靶向TUBA1C基因的siRNA明显、特异性地抑制TUBA1C mRNA的表达水平；培养72h CAOV3实验组细胞增殖能力较空载体转染组和空白对照组明显降低，差异有统计学意义（P＜0.05）；侵袭实验结果提示SKOV3实验组穿膜细胞数量为（0.95±0.12）个/视野，较空载体转染组降低，差异有统计学意义（P＜0.01），CAOV3实验组穿膜细胞数量为（1.13±0.14）个/视野，较空白对照组降低，但差异无统计学意义（P＞0.05）。结论抑制TUBA1C基因过表达可抑制CAOV3、SKOV3细胞的增殖、侵袭能力，有望成为卵巢癌治疗的新靶点。

Silencing over-expression of TUBA1C inhibits proliferation and invasion of human ovarian carcinoma CAOV3, SKOV3 cells

Objective To investigate the effect of silencing the over- expression of alpha- tubulin specific 1c chain（TU-BA1C） gene on the proliferation and invasion of human ovarian carcinoma CAOV3 and SKOV3 cells. Methods TUBAIC se-quencing- targeting siRNA was transfected into human ovarian carcinoma CAOV3 and SKOV3 cells. The expressions of TUBAICmRNA was examined by RT- PCR, the proliferation and invasion ability of CAOV3 and SKOV3 cells were evaluated by CCK- 8 assay and Transwel assay, respectively. Results The TUBA1C sequence- specific siRNA effectively and specifically down- regulated the expression of TUBA1CmRNA. The CCK- 8 results showed that the proliferation of cultured CAOV3 cells in experimental was significantly inhibited compared with those in control and negative transfection groups（P＆lt;0.05）.The penetrat-ing capacity of SKOV3 cells in the experimental group （0.95＆#177;0.12 cells/field） was significantly inhibited compared with those in the negative group（P＆lt;0.01）;however there was no significant differnce in CAOV3 cells between experimental group and negative group. Conclusion Silencing TUBA1C can effectively inhibit the proliferation and invasion of human ovarian carcinoma CAOV3 and SKOV3 cells, suggesting TUBA1C gene may be a potential new target for treatment of ovarian carcinoma.