雌激素治疗对绝经妇女Th1/Th2平衡的影响

目的：探讨雌激素治疗对绝经妇女辅助性T细胞1（Th1）/辅助性T细胞2（Th2）平衡的影响。方法将研究对象分为生育年龄组、绝经组、雌激素治疗组共3组，每组各10例，分离、培养所有研究对象外周血T淋巴细胞，ELISA法测培养液上清液IL-4、IFN-γ质量浓度，并计算IFN-γ/IL-4比值。结果绝经组IFN-γ水平明显高于生育年龄组、IL-4水平显著低于生育年龄组、IFN-γ/IL-4比值显著高于生育年龄组，差异均有统计学意义（P＜0.05或0.01）；雌激素治疗组IL-4水平显著低于生育年龄组、IFN-γ/IL-4比值显著高于生育年龄组，差异均有统计学意义（均P＜0.01）；雌激素治疗组IFN-γ水平明显低于绝经组、IL-4水平显著高于绝经组、IFN-γ/IL-4比值显著低于绝经组，差异均有统计学意义（P＜0.05或0.01）。结论绝经后发生Th1/Th2平衡变化，出现向Th1漂移现象，而雌激素治疗能改善Th1/Th2平衡的偏移。

Effects of estrogen therapy on the Th1/Th2 balance in postmenopausal women

Objective To investigate the effects of estrogen therapy on Th1/Th2 balance in postmenopausal women. Methods The study included 10 women in child- bearing period, 10 postmenopausal women and 10 postmenopausal women with estrogen therapy. The peripheral blood lymphocytes were separated and cultured, the levels of IL- 4 and IFN- γin cultured su-pernatant were measured by ELISA, and ratio of Th1/Th2 （IFN- γ/IL- 4） was calculated. Results The IFN- γ level in post-menopausal women was significantly higher, the IL- 4 level was lower and the IFN- γ/IL- 4 ratio was higher than those in child- bearing period women （P〈0.05 or 0.01）. The IL- 4 level in estrogen therapy group was lower and IFN- γ/IL- 4 ratio was higher than those in child- bearing period group （ both P〈0.01） . The IFN- γlevel was lower, the IL- 4 level was higher and the IFN- γ/IL- 4 ratio in estrogen therapy group was lower than those in postmenopausal group （P〈0.05 or 0.01）. Conclusion The peripheral blood lymphocytes shift towards Th1 in post- menopausal women, and estrogen therapy can regulate the Th1/Th2 bal-ance.