吡格列酮对非酒精性脂肪肝大鼠肝脏氧化应激的影响

目的：观察吡格列酮对高脂饮食诱导的非酒精性脂肪性肝病（NAFLD）大鼠肝脏组织氧化应激的影响。方法将30只SD大鼠随机分为正常组、模型组和吡格列酮组（每组10只）。正常组全程饲以普通饲料，另两组均给予高脂饮食。6周后每组各处死2只大鼠，验证NAFLD造模成功后，给予吡格列酮组大鼠10mg·kg-1·d-1吡格列酮灌胃，正常组、模型组给予相应体积的0.9%氯化钠溶液灌胃。6周后处死并留取肝组织，行病理组织学检查，硫代巴比妥酸法测定丙二醛（MDA）的含量，免疫组织化学法检测肝脏组织血红蛋白氧合酶（HO）-1的表达。结果与模型组相比，吡格列酮组大鼠肝组织病变改善，MDA的含量显著下降，且该组肝脏组织内HO-1表达较正常组、模型组均显著升高（均P＜0.05）。结论吡格列酮可诱导NAFLD大鼠肝脏组织内HO-1的表达，减弱氧化应激，可能是其改善NAFLD病理变化的机制之一。

Effects of pioglitazone on oxidative stress in rats with non-alcoholic fatty liver disease

Objective To investigate the effects of pioglitazone on oxidative stress in rats with high fat diet- induced non- alcoholic fatty liver disease （NAFLD）. Methods Thirty SD rats were randomly divided into normal group, model group and pioglitazone group （n=10 in each）. NAFLS was induced by feeding of high- fat diet in rats of model and pioglitazone groups. Rats in pioglitazone group were gavaged with 10mg/kg of pioglitazone daily;rats in normal and model groups received an equal vol-ume of saline. Two rats from each group were sacrificed at the end of week 6 for pathological examination;and at the end of week 12 al rats were sacrificed. The histological examinations were performed, contents of malondialdehyde （MDA） and heme oxyge-nase- 1 （HO- 1） in liver were determined. Results Compared with model group, the degrees of hepatic steatosis and inflamma-tion were significantly al eviated and contents of MDA in liver were decreased in pioglitazone group（P〈0.05）. The liver HO- 1 lev-els were significantly higher in pioglitazone group than those in normal and model groups（P〈0.05）. Conclusion Pioglitazone can induce the expression of HO- 1 in liver, which is associated with its protective effect on oxidative stress and attenuation of non- alcoholic fatty liver disease in rats.