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| 不同转移潜能的人胰腺癌JF305单克隆细胞亚系的建立 | |
| 引用本文： | 崔宏,何三光,刘永锋,王士勇,王晓华,王宏,戴晓纯,宗阿南,何安光. 不同转移潜能的人胰腺癌JF305单克隆细胞亚系的建立[J]. 中国普外基础与临床杂志, 1999, 6(6): 334-336 |
| 作者姓名： | 崔宏 何三光 刘永锋 王士勇 王晓华 王宏 戴晓纯 宗阿南 何安光 |
| 作者单位： | 1. 中国医科大学第一临床学院普外一科,沈阳,110001 2. 中国医科大学肿瘤研究所胰癌研究室 3. 中国医科大学动物部 |
| 摘 要： | 为研究胰腺癌的转移机理，在我校原建立的人胰腺癌ＪＥ３０５细胞系基础上，利用细胞克隆，细胞电泳，流式细胞依及人癌小鼠原位移植技术，建立了具有不同转移潜能的人胰腺癌ＪＦ３０５单克隆细胞亚系和具有不同转移潜能的人朱癌单克隆细胞亚系裸小鼠原位移植模型。结果表明该单克隆细胞亚系及其肿瘤移植模型的建立，为探讨胰腺癌转移机理提供了较理想的研究方法。 |
| 关 键 词： | 胰腺癌 转移潜能 单克隆细胞亚系 建系 |
THE ESTABLISHMENT OF THE MONOCLONAL CELL SUBLINES WITH DIFFERENT METASTATIC POTENTIAL FROM HUMAN PANCREATIC CANCER JF305 |
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| Cui Hong,He Sanguang,Liu Yongfeng,et al.. THE ESTABLISHMENT OF THE MONOCLONAL CELL SUBLINES WITH DIFFERENT METASTATIC POTENTIAL FROM HUMAN PANCREATIC CANCER JF305[J]. Chinese Journal of Bases and Clinics In General Surgery, 1999, 6(6): 334-336 | |
| Authors: | Cui Hong He Sanguang Liu Yongfeng et al. |
| Affiliation: | Cui Hong,He Sanguang,Liu Yongfeng,et al. First Department of General Surgery,First Clinical College,China Medical University,Shenyang 110001 |
| Abstract: | On the basis of established JF305 cell line from human pancreatic cancer at this university, cell clone technique, cell electrophoresis, flower cytometer, and cancer orthotopically implanted nude mice technique were used to establish the sublines with different metastatic potential from human pancreatic cancer line JF305 and the nude mice model implanted orthotopically with human pancreatic cancer monoclonal sublines with different metastatic potential. The results showed that the monoclonal cell sublines with different metastatic potential from human pancreatic caner JF305 and the nude mice model implanted orthotopically with the sublines, would provided a useful method to study the metastatic mechanism of human pancreatic cancer. |
| Keywords: | Pancreatic neoplasma Cell line Metastatic potential Cancer orthotopically implanted nude mice |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |