白藜芦醇对急性胰腺炎肺损伤大鼠树突状细胞的影响

目的 观察白藜芦醇（resveratrol,Res）对重症急性胰腺炎（SAP）肺损伤大鼠树突状细胞（DC）的影响.方法 36只SD大鼠随机分为三组：假手术组、SAP模型组、Res治疗组（Res 10 mg/kg）,每组各12只.各组SD大鼠于制模后12 h处死,行胰腺及肺组织病理学检查和评分.提取肺树突状细胞,流式细胞仪检测DC表型CD80表达,并检测IL-12的分泌水平.结果 Res组肺和胰腺组织病理学评分分别为（3.49±1.02）和（6.43±1.40）,而SAP组分别为（6.12±2.02）和（11.00±2.58）,Res组评分均显著降低（P＜ 0.05）.SAP组DC细胞表面分子CD80阳性表达率为（45.3±6.7）％,而Res组为（20.1±3.2）％,显著低于SAP组,差异有统计学意义（P＜0.05）;Res组和SAP组DC分泌IL-12浓度分别为（1 676.5±121.8） g/L和（4 436.4±169.8） g/L,Res组IL-12浓度显著低于SAP组,差异有统计学意义（P＜0.05）.结论 Res能显著减轻SAP时肺和胰腺组织损伤,其机制与抑制肺DC活化有关.

Effects of resveratrol on function of dendritic cell in lung of rats with severe acute pancreatitis

Objective To investigate the effect of resveratrol （Res） on dendritic cell in lung of rats with severe acute pancreatitis associated lung injury.Methods Thirty-six rats were divided into three groups：sham group,SAP model group and Res treated group.Rats were sacrificed at 12 h after inducing SAP model.The pathological changes of pancreas and lung were observed,and the level of IL-12 was examined by ELISA method.Results Histopathology scores of lung and pancreas in Res group were significantly lower than that in SAP group [（3.49 ± 1.02） and （6.43 ± 1.4）,（6.12 ± 2.02） and （11.00 t 2.58）,P＜0.05）].The positive rate of the phenotype of CD80 in Res group was （20.1 ± 3.2） ％,which was significantly lower than that in SAP group [（45.3 ± 6.7）％,P ＜0.05].The level of the IL-12 in Res group was （1 676.5 ± 121.8） g/L,which was significantly lower than that in SAP group [（4 436.4 ± 169.8） g/L,P＜0.05].Conclusion Resveratrol can relieve the injury of pancreas and lung in SAP,the mechanism of which may be related with the inhibitory effects of Res on activation of dendritic cell.