SD大鼠Müller细胞的原代培养

目的:原代分离培养SD大鼠Mller细胞,在实验室建立Mller细胞株。方法:采用改良的酶消化法培养新生大鼠的视网膜Mller细胞,免疫组化法进行鉴定。结果:原代培养的Mller细胞胞体狭长,胞浆丰富,经过3~5次传代后逐渐变得胞体宽大,出现微丝和突起。免疫组化显示,95%以上的细胞谷氨酰胺合成酶染色阳性。结论:改良的酶消化法是培养视网膜Mller细胞的简单有效的方法。

Primary culture of Sprague-Dawley rat mller cells

AIM: To culture mller cell and established cell strain in our laboratory. ·METHODS: The retina was obtained from neonate rat and mller cells were isolated by Modified enzyme digestion. The cultured mller cell was identified by immuocytochemistry, and was observed under inverse phase microscope. ·RESULTS: The cultured mller cell had large cell body and abundant kytoplasm. The body became wider and microfilament and cytodendrite appeared after 3-5 passage. More than 95% of the cells were positive stained for the glutamine synthetase. ·CONCLUSION: The modified enzyme digestion method is a simple and effective way for culturing mller cells.