乙型肝炎后肝硬化中医证候与白细胞介素10基因多态性的关联

目的：探讨白细胞介素10（interleukin-10, IL-10）基因的单核苷酸多态性（single-nucleotide polymorphism, SNP）与乙型病毒性肝炎（乙肝）后肝硬化中医证候的关联。 方法：运用聚合酶链反应限制性片段长度多态性（polymerase chain reaction-restriction fragment length polymorphism, PCR-RFLP）和聚合酶链反应连接酶检测（polymerase chain reaction-ligase detection reaction, PCR-LDR）相结合的测序方法，检测226例乙肝后肝硬化患者IL-10-592 A/C、-819 C/T和-1082 G/A位点的多态性，分析其基因型和等位基因频率的分布，以及这3个SNP位点与乙肝后肝硬化中医证候之间的关系。 结果：IL-10-819位点C等位基因在脾虚湿盛证出现的频率明显高于非脾虚湿盛证（P＜0.001），TT基因型在肝气郁结证出现的频率明显高于非肝气郁结证（P＜0.05）。IL-10-592 A/C和-1082 G/A位点的多态性与乙肝后肝硬化中医证候之间未发现有显著的关联（P〉0.05）。 结论：IL-10-819位点C等位基因可能与乙肝后肝硬化患者形成脾虚湿盛证相关，而其TT基因型可能与肝硬化患者形成肝气郁结证相关。

Relationship between gene polymorphisms of interleukin-10 and syndrome types of traditional Chinese medicine in posthepatitis B cirrhosis

Objective： To investigate the relationship between single-nucleotide polymorphisms （SNPs） of interleukin-10 （IL-10） and syndrome types of traditional Chinese medicine （TCM） in posthepatitis B cirrhosis.
Methods： The genotypes of IL-10-592 A/C, -819 C/T and -1082 G/A sites were determined by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）, and polymerase chain reaction-ligase detection reaction （PCR-LDR） combined with the sequencing analysis in 226 cases of posthepatitis B cirrhosis. The genotype and allele frequency distribution, and the relationship between the SNPs and TCM syndromes were analyzed.
Results： The frequency of allele C at IL-10-819 point in spleen deficiency with overabundance of dampness syndrome was significantly higher than that in non-spleen deficiency with overabundance of dampness （P〈0.01） syndrome, and genotype TT in liver stagnation syndrome was significantly higher than that in non-liver stagnation syndrome （P〈0.05）. There was no significant relationship between the polymorphisms of IL-10-592 A/C, -1082 G/A and the TCM syndromes in posthepatitis B cirrhosis （P〉0.05）.
Conclusion： In patients with posthepatitis B cirrhosis, allele C in IL-10-819 locus may be related to spleen deficiency with overabundance of dampness syndrome, and TT genotype in IL-10-819 locus may be related to liver stagnation syndrome.