虎金颗粒剂提取工艺的研究

【目的】探讨虎金颗粒剂的最佳提取工艺路线。【方法】采用正交设计，以总大黄素、总蒽醌含量为醇提工艺指标，考察乙醇浓度、溶媒用量、提取时间等因素的最佳工艺务件；以总多糖含量为水提工艺指标，考察浸泡时间、水的用量、提取时间等因素的最佳工艺条件。【结果】醇提最佳工艺为：245mL体积分数为70％乙醇提取2h，共2次；水提最佳工艺为：80mL水，不用浸泡，提取1．5h，共3次。【结论】该提取工艺合理的，可行，为选择虎金颗粒剂的提取条件提供了实验依据。

Studies on Extracting Process of Hujin Granules

[Objective] To optimize the conditions of the extracting process of Hujin Granules. [Methods] The orthogonal design was applied. With the total emodin and the total anthraquinone (TA) content as the parameters for the alcohol-extraction, the concentration of alcohol, the volume of solvent and the extracting time were used for optimization of alcohol-extraction. With the total polysaccharide (TP) as the parameters for water-extraction, the soaking time, the volume of water and the extracting time were used for optimization of water-extraction. [Results] The optimum conditions of alcohol-extraction were: extracting with 70% alcohol 245 mL for 2 hours and extracting twice. The optimum conditions of water-extraction were: extracting with 80 mL water (not for soaking) for 1.5 hours, extracting 3 times. [Conclusion] The results indicate that the extracting process is rational and feasible, and can provide evidence for the extracting process of Hujin Granules.