维生素B_(12)对体外乙醇诱发小鼠胚胎畸变的影响

目的探讨维生素B12(VB12)对乙醇发育毒性的干预作用。方法采用植入后全胚胎培养技术(WEC),将GD8.5的ICR小鼠胚胎用4.0g/L乙醇处理,同时用不同浓度VB12(10-6、10-5和10-4mol/L)干预。在大鼠即刻离心血清中旋转培养48h后对小鼠胚胎生长发育和组织器官形态分化进行评分。结果应用不同浓度VB12干预后,反映胚胎生长发育和组织器官形态分化的指标得分与阳性对照组相比,均有不同程度的提高。其中,10-5mol/LVB12干预组对胚胎生长发育干预得分总和最高,颅臀长、头长、体节数与阳性对照组相比差异均有显著性(P<0.05)。10-4mol/LVB12干预组对胚胎组织器官形态分化干预得分总和最高,除尿囊、心脏、上颌突、下颌突指标外,其他与阳性对照组相比差异均有显著性(P<0.05)。结论VB12有拮抗乙醇发育毒性的作用。

Effects of vitamin B12 on ethanol-induced mouse embryonic abnormality in vitro

Objective To evaluate the developmental improvement of vitamin B_ 12 on ethanol-induced embryonic abnormality in vitro. Methods Whole embryo culture system was used in this study. Early somite stage (GD8.5) mouse embryo was explanted and cultured in rat serum medium (immediately centrifugal serum, ICS) with vitamin B_ 12 (0, 10~ -6 , 10~ -5 and 10~ -4 mol/L) and ethanol (4.0g/L) for 48h to evaluate the embryo development and morphological differentiation. Results Developments of crown-rump length, head length and somite number were significantly improved at the concentration of 10~ -5 mol/L vitamin B_ 12 (P<0.05) and the indexes of embryonic growth and development except allantoid, heart, maxillary and mandible significantly increased at the concentration of 10~-4 mol/L vitamin B_12 (P<0.05), which were all compared with the positive group. Conclusion Vitamin B_12 could improve ethanol-induced developmental toxicity in mice in vitro.