转化生长因子β1基因多态性与慢性肾衰竭的相关性研究

目的 探讨转化生长因子(TGF-β1)T869C基因多态性与慢性肾衰竭的相关性.方法 将入选该研究的79例慢性肾小球肾炎患者(21例肾功能正常,58例肾功能异常)和70例健康体检者分为慢性肾小球肾炎肾功能代偿组、慢性肾小球肾炎肾功能失代偿组和健康对照组.用序列特异性引物聚合酶链(PCR-SSP)技术检测TGF-β1 T...

Association study of gene polymorphism of transforming growth factor-beta 1 and chronic renal failure

Objective To investigate the relationship between polymorphism of transforming growth factor-beta 1(TGF-β1)T869C and chronic renal failure(CRF).Methods Seventy-nine patients with chronic glomerulonephritis(21 of patients with normal renal function and 58 with CRF)and 70 healthy controls were included in the present study.All of them were divided into three groups:control group、chronic glomerulonephritis with normal renal function group、and chronic glomerulonephritis with CRF group.The polymorphism of TGF-βl gene T869C was analyzed by sequence specific primers-polymerase chain reaction method.Results No significant differences in distributions of TGF-β1 T869C genotype and allele frequencies were found between the chronic glomerulonephritis with normal renal function group(TT:TC:CC= 42.9%:38.1%:9.0%,T:C=61.9%:38.1%)and the control group(TT:TC:CC= 34.3%:50%:15.7%,T:C=59.3%:40.7%)(P>0.05).However,the TGF-β1 T869C polymorphism in the chronic glomerulonephritis with CRF group(TT:TC:CC= 13.8%:50.0%:36.2%,T:C=38.8%:61.2%)was significant different from control group and chronic glomerulonephritis with normal renal function group.The frequencies of C allele and CC homozygote in the chronic glomerulonephritis with CRF group were significantly higher than the other two groups(P<0.05).The relative risk suffered from CRF of C allele was 2.297 times to that of the T allele(OR=2.297,95%CI:1.395~3.783).Conclusion TGF-β1 gene T869C polymorphism is associated with CRF.C allele and CC genotype may be two genetic markers for the genesis and development of CRF.