| BDNF基因修饰真皮多能干细胞移植对脊髓损伤的修复作用 |
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| 引用本文： | 宗兆文,张连阳,沈岳,郭庆山,任永川,冉新泽,史春梦,程天民. BDNF基因修饰真皮多能干细胞移植对脊髓损伤的修复作用[J]. 重庆医学, 2011, 40(29): 2913-2914,2917,2908. DOI: 10.3969/j.issn.1671-8348.2011.29.001 |
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| 作者姓名： | 宗兆文 张连阳 沈岳 郭庆山 任永川 冉新泽 史春梦 程天民 |
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| 作者单位： | 1. 第三军医大学大坪医院/野战外科研究所全军战创伤中心创伤科,重庆,400042
2. 第三军医大学全军复合伤研究所国家创伤、烧伤与复合伤重点实验室复合伤分室,重庆,400038 |
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| 基金项目： | 创伤烧伤与复合伤国家重点实验室自主课题，重庆市自然科学基金重点项目 |
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| 摘 要： | 目的 观察脑源性神经营养因子(BDNF)基因修饰的真皮多能干细胞(dMSCs)移植后对损伤脊髓的修复作用.方法 将72只大鼠采用改良Allen 重物打击法制备脊髓损伤模型,将其随机均分为A组[接受BDNF腺病毒表达载体(Adv-BDNF)感染的dMSCs移植]、B组(接受未感染Adv-BDNF的dMSCs移植)、C组(...
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| 关 键 词： | 脊髓损伤 脑源性神经营养因子 干细胞移植 大鼠 |
Role of transplantation of BDNF gene-modified dermal multipotent stem cells in repairment of spinal cord injury |
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| Zong Zhaowen,Zhang Lianyang,Shen Yue,Guo Qingshan,Ren Yongchuan,Ran Xinze,Shi Chunmeng,Chen Tianming. Role of transplantation of BDNF gene-modified dermal multipotent stem cells in repairment of spinal cord injury[J]. Chongqing Medical Journal, 2011, 40(29): 2913-2914,2917,2908. DOI: 10.3969/j.issn.1671-8348.2011.29.001 |
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| Authors: | Zong Zhaowen Zhang Lianyang Shen Yue Guo Qingshan Ren Yongchuan Ran Xinze Shi Chunmeng Chen Tianming |
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| Affiliation: | Zong Zhaowen1,Zhang Lianyang1,Shen Yue1,Guo Qingshan1,Ren Yongchuan1,Ran Xinze2,Shi Chunmeng2,Chen Tianming2(1.Department of Traumatology,Trauma Center,Institute of Surgery Research,Daping Hospital,the Third Military Medical University,Chongqing 400042,China,2.Combined Injury Branch,State Key Laboratory of Trauma,Burns and Combined Injury,Institute of Combined Injury of PLA,Chongqing 400038,China) |
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| Abstract: | Objective To observe the repair effects of transplantation of dermal multipotent stem cells(dMSCs)modified by brain-derived neurotrophic factor(BDNF)gene on spinal cord injury.Methods 72 rats was used to establish spinal cord injury models with improved Allen′s falling strike method,and then they were randomly divided into 4 groups:group A[accepted transplantation of dMSCs infected by adenovirus vector of BDNF(Adv-BDNF)],group B(accepted transplantation of dMSCs not infected by Adv-BDNF),group C(injection with only Adv-BDNF)and group D(only spinal cord injury).Immunofluorescence histochemistry and flow cytometry were employed to detect the expression of BDNF and apoptosis of cells in the site of spinal cord injury,respectively.BBB neurological behavior score method was used to assess neurofunctional recovery of injuried rats.Results Expression of BDNF in the site of spinal cord injury in group A and C were higher than those in group B and D;Compared with group B,C,D group,group A showed the lowest percentage of apoptotic cells in the site of spinal cord injury and the best neurofunctional recovery.Conclusion BDNF and dMSCs transplantation can synergistically promote the functional recovery of injuried spinal cord. |
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| Keywords: | spinal cord injuries brain-derived neurotrophic factor stem cell transplantation rats |
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