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| 负载miR-138复制缺陷型腺病毒对hTERT表达的影响 | |
| 引用本文： | 刘志鹏,王宗华,梁光萍,熊晓峰,范亚川,邹利全,王洪斌,陈陵,李学成. 负载miR-138复制缺陷型腺病毒对hTERT表达的影响[J]. 重庆医学, 2011, 40(29): 2947-2948,2951. DOI: 10.3969/j.issn.1671-8348.2011.29.013 |
| 作者姓名： | 刘志鹏 王宗华 梁光萍 熊晓峰 范亚川 邹利全 王洪斌 陈陵 李学成 |
| 作者单位： | 1. 中国人民解放军第三二四医院消化内科,重庆,400020 2. 第三军医大学护理学院,重庆,400038 3. 第三军医大学西南医院烧伤研究所,重庆,400038 |
| 基金项目： | 重庆市自然科学基金资助 |
| 摘 要： | 目的探讨以复制缺陷型腺病毒为载体,上调人端粒酶逆转录酶(hTERT)调控相关miR-138表达丰度后,能否有效抑制靶基因hTERT的表达。方法 将负载hTERT调控相关miR-138的复制缺陷型腺病毒以200∶1的感染复数(MOI),感染人胃癌BGC-823细胞;采用实时荧光定量PCR(real-time PCR)检测BGC-823细胞内miR-138和hTERT mRNA的表达丰度;Western Blot检测hTERT蛋白表达。结果 与阴性对照腺病毒相比,负载miR-138的腺病毒感染BGC-823细胞后,可显著提高miR-138表达丰度(P<0.05);而未感染腺病毒的空白对照组与阴性对照组相比差异无统计学意义(P>0.05)。与感染阴性对照腺病毒的BGC-823细胞相比,感染负载人miR-138腺病毒(Ad-miR138)的BGC-823细胞内hTERT蛋白表达有所下调。结论 以复制缺陷型腺病毒为载体,可有效上调hTERT调控相关miR-138的表达丰度,进而可有效抑制miR-138靶基因hTERT的表达水平。 |
| 关 键 词： | RNA指导的DNA聚合酶 胃肿瘤 复制缺陷型腺病毒载体 |
Effects of replication-deficient adenovirus carrying miR-138 on the hTERT expression |
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| Liu Zhipeng,Wang Zonghua,Liang Guangping,Xiong Xiaofeng,Fan Yachuan,Zou Liquan,Wang Hongbin,Chen Ling,Li Xuecheng. Effects of replication-deficient adenovirus carrying miR-138 on the hTERT expression[J]. Chongqing Medical Journal, 2011, 40(29): 2947-2948,2951. DOI: 10.3969/j.issn.1671-8348.2011.29.013 | |
| Authors: | Liu Zhipeng Wang Zonghua Liang Guangping Xiong Xiaofeng Fan Yachuan Zou Liquan Wang Hongbin Chen Ling Li Xuecheng |
| Affiliation: | 1△(1.Gastrointestinal Department,324 Hospital of PLA,Chongqing 400020,China;2 Nursing School,Third Military Medical University;3.Burning Institution,Southwest Hospital,Third Military Medical University,Chongqing 400038,China) |
| Abstract: | Objective To identify effects of the replication-deficient recombinant adenovirus carrying miR-138 on the expression level miR-138 and the target gene hTERT.Methods The human gastric cancer cell line BGC-823 were infected with the replication-deficient recombinant adenovirus carrying miR-138 by the MOI of 200:1.The expression levels of miR-138 and hTERT mRNA were inuestigated by real-time PCR.The expression of hTERT protein was detected by western blot.Results Compared to the negative control,after infectin... |
| Keywords: | RNA-directed DNA polymerase stomach neoplasms replication-deficient adenoviral vector |
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