| 嗜肺军团菌环介导等温扩增检测方法的建立及应用简 |
| |
| 引用本文： | 张晓航,陆晔,周娴,周启明,李俊,田桢干,章琪,吴凡,杜正平,方筠.嗜肺军团菌环介导等温扩增检测方法的建立及应用简[J].中国国境卫生检疫杂志,2014(1):6-11,15. |
| |
| 作者姓名： | 张晓航 陆晔 周娴 周启明 李俊 田桢干 章琪 吴凡 杜正平 方筠 |
| |
| 作者单位： | [1]上海国际旅行卫生保健中心,上海200335 [2]广州华峰生物科技有限公司,上海200335 |
| |
| 基金项目： | 上海检验检疫局科研基金项目（HK029-2011） |
| |
| 摘 要： | 目的建立一种灵敏、快速、安全、方便适合口岸现场应用的嗜肺军团菌检测方法。方法分析嗜肺军团菌基因组序列,选择特异性基因片段设计引物,建立环介导等温扩增（loop mediated isothermal amplification,LMAP）方法,并优化反应条件。结果建立的方法特异性好,灵敏度可达103cfu/ml,检测能力与荧光定量PCR法相当。结论应用环介导恒温扩增技术建立的嗜肺军团菌检测方法,灵敏度高,特异性好,适合现场快速检测。
|
| 关 键 词： | 嗜肺军团菌 环介导等温扩增法 检测 应用 |
Establishment and application on detection of Legionella pneumophila using LAMP technology |
| |
| ZHANG Xiao-hang,LU Ye,ZHOU Xian,ZHOU Qi-ming,LI Jun,TIAN Zhen-gan,ZHANG Qi,WU Fan,DU Zheng-ping,FANG Yun.Establishment and application on detection of Legionella pneumophila using LAMP technology[J].Chinese Journal of Frontier Health and Quarantine,2014(1):6-11,15. |
| |
| Authors: | ZHANG Xiao-hang LU Ye ZHOU Xian ZHOU Qi-ming LI Jun TIAN Zhen-gan ZHANG Qi WU Fan DU Zheng-ping FANG Yun |
| |
| Institution: | ＊Shanghai International Travel Healthcare Center, Shanghai 200335, China |
| |
| Abstract: | Objective To establish a sensitive, rapid, safe and convenient Legionella pneumophila detection methods for applications on port. Methods Legionella pneumophila genome sequence was, analyzed specific gene fragment primers was selected to establish loop-mediated isothermal amplification （loop mediated isothermal amplification LMAP） method, and the reaction condition were optimized. Results Specificity of the established method was good, the sensitivity was 103cfu/ml. The detection capability corresponded to quantitative PCR. Conclusion The Legionella pneumophila LAMP technology detection methods has high sensitivity, specificity, suitable for on-site rapid detection. |
| |
| Keywords: | Legionella pneumophila loop-mediated isothermal amplification （LAMP） Detect Application |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
