大黄-桃仁配伍对粘连性肠梗阻大鼠炎性细胞因子及肠黏膜屏障的影响

目的:观察大黄-桃仁对术后粘连性肠梗阻模型大鼠炎性细胞因子水平及肠黏膜屏障的影响，探讨其作用机制。方法:将60只SD大鼠按随机数字表法分为正常组，假手术组，模型组，大黄-桃仁低、中、高剂量组，每组10只。除正常组和假手术组外，其余各组大鼠制备术后粘连性肠梗阻模型。造模后第1天开始，大黄-桃仁低、中、高剂量组灌胃0.2、...

Effect of Rhei Radix et Rhizoma and Persicae Semen on inflammatory cytokines and intestinal mucosal barrier in rats with adhesive intestinal obstruction

Objective To observe the effect of Rhei Radix et Rhizoma and Persicae Semen on inflammatory cytokines and intestinal mucosal barrier in rats with postoperative adhesive intestinal obstruction,and to explore its mechanism.Methods Sixty SD rats were randomly divided into normal group,sham operation group,model group,low,medium and high dose of Rhei Radix et Rhizoma and Persicae Semen group.Except for the normal group and the sham operation group,and the other animals groups were established the model of postoperative adhesive intestinal obstruction.Rhei Radix et Rhizoma and Persicae Semen low,medium and high dose groups were perfused with Rhei Radix et Rhizoma and Persicae Semen decoction with the concentration of 0.2,0.6 and 1.8 g/ml,the normal group,sham operation group and model group were gavaged with equal volume of sterile saline from the first day after the operation,once a day.After corresponding treatment,the adhesion score was observed on the 7th day after the operation,the contents of interleukin-1β(IL-1β),D-lactic acid(D-LA),diamine oxidase(DAO)and vascular endotoxin(ET)in serum were detected by ELISA method,and the expression of SIgA,CD4⁺T and CD8⁺T cells in intestinal mucosa were assessed by immunohistochemical method.Results Compared to the model group,the adhesion score in the low,medium and high dose of Rhei Radix et Rhizoma and Persicae Semen group significantly decreased(P<0.05),the level of serum IL-1β(8.66±1.07)ng/L,(8.15±1.23)ng/L,(7.99±1.11)ng/L vs.(14.08±2.54)ng/L]significantly decreased(P<0.01),the expression of SIgA(1.38±0.15,2.87±1.17,2.79±0.80 vs.0.65±0.12)in intestinal mucosa in the low,medium and high dose of Rhei Radix et Rhizoma and Persicae Semen group significantly increased(P<0.01).The levels of serum D-LA(8.57±1.73)mg/L,(7.13±1.75)mg/L vs.(14.58±2.81)mg/L],ET(77.39±6.83)mg/ml,(50.49±7.80)mg/ml vs.(138.22±7.79)mg/ml]significantly decreased(P<0.01),the expression of CD4⁺T(2.61±0.83,2.91±1.62 vs.1.15±0.98)and CD8⁺T(2.88±0.69,3.01±1.86 vs.1.26±0.74)cells in intestinal mucosa in the medium and high dose of Rhei Radix et Rhizoma and Persicae Semen group significantly increased(P<0.01).Conclusion Rhei Radix et Rhizoma and Persicae Semen decoction can improve intestinal mucosal permeability,protect intestinal mucosal immune barrier and reduce inflammatory reaction in the treatment of adhesive intestinal obstruction.